Mitochondrial fission proteins regulate programmed cell death in yeast. Export

@article{citeulike:3019524, abstract = {The possibility that single-cell organisms undergo programmed cell death has been questioned in part because they lack several key components of the mammalian cell death machinery. However, yeast encode a homolog of human Drp1, a mitochondrial fission protein that was shown previously to promote mammalian cell death and the excessive mitochondrial fragmentation characteristic of apoptotic mammalian cells. In support of a primordial origin of programmed cell death involving mitochondria, we found that the Saccharomyces cerevisiae homolog of human Drp1, Dnm1, promotes mitochondrial fragmentation/degradation and cell death following treatment with several death stimuli. Two Dnm1-interacting factors also regulate yeast cell death. The WD40 repeat protein Mdv1/Net2 promotes cell death, consistent with its role in mitochondrial fission. In contrast to its fission function in healthy cells, Fis1 unexpectedly inhibits Dnm1-mediated mitochondrial fission and cysteine protease-dependent cell death in yeast. Furthermore, the ability of yeast Fis1 to inhibit mitochondrial fission and cell death can be functionally replaced by human Bcl-2 and Bcl-xL. Together, these findings indicate that yeast and mammalian cells have a conserved programmed death pathway regulated by a common molecular component, Drp1/Dnm1, that is inhibited by a Bcl-2-like function.}, author = {Fannjiang, Yihru and Cheng, Wen-Chih C. and Lee, Sarah J. and Qi, Bing and Pevsner, Jonathan and McCaffery, J. Michael and Hill, R. Blake and Basa\~{n}ez, Gorka and Hardwick, J. Marie}, citeulike-article-id = {3019524}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gad.1247904}, citeulike-linkout-1 = {http://www.genesdev.org/cgi/content/abstract/18/22/2785}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15520274}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15520274}, day = {15}, doi = {10.1101/gad.1247904}, issn = {0890-9369}, journal = {Genes \& development}, keywords = {apoptosis, cit1}, month = {November}, number = {22}, pages = {2785--2797}, posted-at = {2008-07-18 20:51:04}, priority = {2}, title = {Mitochondrial fission proteins regulate programmed cell death in yeast.}, url = {http://dx.doi.org/10.1101/gad.1247904}, volume = {18}, year = {2004} }

TY - JOUR ID - citeulike:3019524 L3 - citeulike-article-id:3019524 N2 - The possibility that single-cell organisms undergo programmed cell death has been questioned in part because they lack several key components of the mammalian cell death machinery. However, yeast encode a homolog of human Drp1, a mitochondrial fission protein that was shown previously to promote mammalian cell death and the excessive mitochondrial fragmentation characteristic of apoptotic mammalian cells. In support of a primordial origin of programmed cell death involving mitochondria, we found that the Saccharomyces cerevisiae homolog of human Drp1, Dnm1, promotes mitochondrial fragmentation/degradation and cell death following treatment with several death stimuli. Two Dnm1-interacting factors also regulate yeast cell death. The WD40 repeat protein Mdv1/Net2 promotes cell death, consistent with its role in mitochondrial fission. In contrast to its fission function in healthy cells, Fis1 unexpectedly inhibits Dnm1-mediated mitochondrial fission and cysteine protease-dependent cell death in yeast. Furthermore, the ability of yeast Fis1 to inhibit mitochondrial fission and cell death can be functionally replaced by human Bcl-2 and Bcl-xL. Together, these findings indicate that yeast and mammalian cells have a conserved programmed death pathway regulated by a common molecular component, Drp1/Dnm1, that is inhibited by a Bcl-2-like function. IS - 22 JF - Genes & development SN - 0890-9369 EP - 2797 TI - Mitochondrial fission proteins regulate programmed cell death in yeast. VL - 18 SP - 2785 KW - apoptosis KW - cit1 AU - Fannjiang, Yihru AU - Cheng, Wen-Chih AU - Lee, Sarah AU - Qi, Bing AU - Pevsner, Jonathan AU - McCaffery, Michael AU - Hill, Blake AU - Basañez, Gorka AU - Hardwick, Marie PY - 2004/11/15/ UR - http://dx.doi.org/10.1101/gad.1247904 ER -