Mechanically Activated Integrin Switch Controls alpha5beta1 Function Export

@article{09science323_friedland_boettiger, abstract = {The cytoskeleton, integrin-mediated adhesion, and substrate stiffness control a common set of cell functions required for development and homeostasis that are often deranged in cancer. The connection between these mechanical elements and chemical signaling processes is not known. Here, we show that {alpha}5{beta}1 integrin switches between relaxed and tensioned states in response to myosin II-generated cytoskeletal force. Force combines with extracellular matrix stiffness to generate tension that triggers the integrin switch. This switch directly controls the {alpha}5{beta}1-fibronectin bond strength through engaging the synergy site in fibronectin and is required to generate signals through phosphorylation of focal adhesion kinase. In the context of tissues, this integrin switch connects cytoskeleton and extracellular matrix mechanics to adhesion-dependent motility and signaling pathways. 10.1126/science.1168441}, author = {Friedland, Julie C. and Lee, Mark H. and Boettiger, David}, citeulike-article-id = {4043517}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1168441}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/323/5914/642}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19179533}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19179533}, day = {30}, doi = {10.1126/science.1168441}, journal = {Science}, keywords = {gjss}, month = {January}, number = {5914}, pages = {642--644}, posted-at = {2009-04-07 11:57:40}, priority = {2}, title = {Mechanically Activated Integrin Switch Controls {alpha}5{beta}1 Function}, url = {http://dx.doi.org/10.1126/science.1168441}, volume = {323}, year = {2009} }

TY - JOUR ID - 09science323_friedland_boettiger L3 - citeulike-article-id:4043517 N2 - The cytoskeleton, integrin-mediated adhesion, and substrate stiffness control a common set of cell functions required for development and homeostasis that are often deranged in cancer. The connection between these mechanical elements and chemical signaling processes is not known. Here, we show that {alpha}5{beta}1 integrin switches between relaxed and tensioned states in response to myosin II-generated cytoskeletal force. Force combines with extracellular matrix stiffness to generate tension that triggers the integrin switch. This switch directly controls the {alpha}5{beta}1-fibronectin bond strength through engaging the synergy site in fibronectin and is required to generate signals through phosphorylation of focal adhesion kinase. In the context of tissues, this integrin switch connects cytoskeleton and extracellular matrix mechanics to adhesion-dependent motility and signaling pathways. 10.1126/science.1168441 IS - 5914 JF - Science EP - 644 TI - Mechanically Activated Integrin Switch Controls {alpha}5{beta}1 Function VL - 323 SP - 642 KW - gjss AU - Friedland, Julie AU - Lee, Mark AU - Boettiger, David PY - 2009/01/30/ UR - http://dx.doi.org/10.1126/science.1168441 ER -