Acidic Sphingomyelinase Mediates Entry of N. gonorrhoeae into Nonphagocytic Cells Export

@article{citeulike:3177477, abstract = {Invasion of human mucosal cells by N. gonorrhoeae via the binding to heparansulfate proteoglycan receptors is considered a crucial event of the infection. Using different human epithelial cells and primary fibroblasts, we show here an activation of the phosphatidylcholine-specific phospholipase C (PC-PLC) and acidic sphingomyelinase (ASM) by N. gonorrhoeae, resulting in the release of diacylglycerol and ceramide. Genetic and/or pharmacological blockade of ASM and PC-PLC cause inhibition of cellular invasion by N. gonorrhoeae. Complementation of ASM-deficient fibroblasts from Niemann-Pick disease patients restored N. gonorrhoeae-induced signaling and entry processes. The activation of PC-PLC and ASM, therefore, is an essential requirement for the entry of N. gonorrhoeae into distinct nonphagocytic human cell types including several epithelial cells and primary fibroblasts.}, author = {Grassm\'{e}, Heike and Gulbins, Erich and Brenner, Birgit and Ferlinz, Klaus and Sandhoff, Konrad and Harzer, Klaus and Lang, Florian and Meyer, Thomas F.}, citeulike-article-id = {3177477}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0092-8674(00)80448-1}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WSN-418PXJ2-8/2/ad38277894ad091a655d45e5c2f113d1}, day = {28}, doi = {10.1016/S0092-8674(00)80448-1}, journal = {Cell}, keywords = {endocytosis, gonorrhoeae, hspg, membrane, sphingomyelinase}, month = {November}, number = {5}, pages = {605--615}, posted-at = {2008-09-01 05:51:01}, priority = {2}, title = {Acidic Sphingomyelinase Mediates Entry of N. gonorrhoeae into Nonphagocytic Cells}, url = {http://dx.doi.org/10.1016/S0092-8674(00)80448-1}, volume = {91}, year = {1997} }

TY - JOUR ID - citeulike:3177477 L3 - citeulike-article-id:3177477 N2 - Invasion of human mucosal cells by N. gonorrhoeae via the binding to heparansulfate proteoglycan receptors is considered a crucial event of the infection. Using different human epithelial cells and primary fibroblasts, we show here an activation of the phosphatidylcholine-specific phospholipase C (PC-PLC) and acidic sphingomyelinase (ASM) by N. gonorrhoeae, resulting in the release of diacylglycerol and ceramide. Genetic and/or pharmacological blockade of ASM and PC-PLC cause inhibition of cellular invasion by N. gonorrhoeae. Complementation of ASM-deficient fibroblasts from Niemann-Pick disease patients restored N. gonorrhoeae-induced signaling and entry processes. The activation of PC-PLC and ASM, therefore, is an essential requirement for the entry of N. gonorrhoeae into distinct nonphagocytic human cell types including several epithelial cells and primary fibroblasts. IS - 5 JF - Cell EP - 615 TI - Acidic Sphingomyelinase Mediates Entry of N. gonorrhoeae into Nonphagocytic Cells VL - 91 SP - 605 KW - endocytosis KW - gonorrhoeae KW - hspg KW - membrane KW - sphingomyelinase AU - Grassmé, Heike AU - Gulbins, Erich AU - Brenner, Birgit AU - Ferlinz, Klaus AU - Sandhoff, Konrad AU - Harzer, Klaus AU - Lang, Florian AU - Meyer, Thomas PY - 1997/11/28/ UR - http://dx.doi.org/10.1016/S0092-8674(00)80448-1 ER -