Footprinting: A method for determining the sequence selectivity, affinity and kinetics of DNA-binding ligands Export

@article{hampshire_footprinting:method_2007, abstract = {Footprinting is a simple method for assessing the sequence selectivity of DNA-binding ligands. The method is based on the ability of the ligand to protect DNA from cleavage at its binding site. This review describes the use of DNase I and hydroxyl radicals, the most commonly used footprinting probes, in footprinting experiments. The success of a footprinting experiment depends on using an appropriate DNA substrate and we describe how these can best be chosen or designed. Although footprinting was originally developed for assessing a ligand's sequence selectivity, it can also be employed to estimate the binding strength (quantitative footprinting) and to assess the association and dissociation rate constants for slow binding reactions.}, author = {Hampshire, Andrew J. and Rusling, David A. and Victoria and Fox, Keith R.}, citeulike-article-id = {3508593}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ymeth.2007.01.002}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WN5-4NKP758-4/2/51884a9596c878afead2d38789d5e824}, doi = {10.1016/j.ymeth.2007.01.002}, journal = {Methods}, keywords = {bindingdnase, dna, footprinting, ifootprintinghydroxyl, radicalssequence, review, selectivity}, month = {June}, number = {2}, pages = {128--140}, posted-at = {2008-11-13 13:14:45}, priority = {2}, title = {Footprinting: A method for determining the sequence selectivity, affinity and kinetics of DNA-binding ligands}, url = {http://dx.doi.org/10.1016/j.ymeth.2007.01.002}, volume = {42}, year = {2007} }

TY - JOUR ID - hampshire_footprinting:method_2007 L3 - citeulike-article-id:3508593 N2 - Footprinting is a simple method for assessing the sequence selectivity of DNA-binding ligands. The method is based on the ability of the ligand to protect DNA from cleavage at its binding site. This review describes the use of DNase I and hydroxyl radicals, the most commonly used footprinting probes, in footprinting experiments. The success of a footprinting experiment depends on using an appropriate DNA substrate and we describe how these can best be chosen or designed. Although footprinting was originally developed for assessing a ligand's sequence selectivity, it can also be employed to estimate the binding strength (quantitative footprinting) and to assess the association and dissociation rate constants for slow binding reactions. IS - 2 JF - Methods EP - 140 TI - Footprinting: A method for determining the sequence selectivity, affinity and kinetics of DNA-binding ligands VL - 42 SP - 128 KW - bindingdnase KW - dna KW - footprinting KW - ifootprintinghydroxyl KW - radicalssequence KW - review KW - selectivity AU - Hampshire, Andrew AU - Rusling, David AU - Victoria AU - Fox, Keith PY - 2007/June// UR - http://dx.doi.org/10.1016/j.ymeth.2007.01.002 ER -