Arabidopsis Phyllotaxis Is Controlled by the Methyl-Esterification Status of Cell-Wall Pectins Export

@article{DOI - 10.1016/j.cub.2008.10.065, abstract = {Plant organs are produced from meristems in a characteristic pattern. This pattern, referred to as phyllotaxis, is thought to be generated by local gradients of an information molecule, auxin [16]. Some studies propose a key role for the mechanical properties of the cell walls in the control of organ outgrowth [712]. A major cell-wall component is the linear ±-1-4-linked D-GalAp pectic polysaccharide homogalacturonan (HG), which plays a key role in cell-to-cell cohesion [13, 14]. HG is deposited in the cell wall in a highly (70\%80\%) methyl-esterified form and is subsequently de-methyl-esterified by pectin methyl-esterases (PME, EC 3.1.1.11). PME activity is itself regulated by endogenous PME inhibitor (PMEI) proteins [15]. PME action modulates cell-wall-matrix properties and plays a role in the control of cell growth [1618]. Here, we show that the formation of flower primordia in the Arabidopsis shoot apical meristem is accompanied by the de-methyl-esterification of pectic polysaccharides in the cell walls. In addition, experimental perturbation of the methyl-esterification status of pectins within the meristem dramatically alters the phyllotactic pattern. These results demonstrate that regulated de-methyl-esterification of pectins is a key event in the outgrowth of primordia and possibly also in phyllotactic patterning.}, author = {Peaucelle, Alexis and Louvet, Romain and Johansen, Jorunn N. and H\"{o}fte, Herman and Laufs, Patrick and Pelloux, J\'{e}rome and Mouille, Gr\'{e}gory}, citeulike-article-id = {3822231}, citeulike-linkout-0 = {http://linkinghub.elsevier.com/retrieve/pii/S0960982208015340}, day = {23}, issn = {0960-9822}, keywords = {cellbio, devbio, phyllotaxis}, month = {December}, number = {24}, pages = {1943--1948}, posted-at = {2008-12-23 16:51:10}, priority = {2}, title = {Arabidopsis Phyllotaxis Is Controlled by the Methyl-Esterification Status of Cell-Wall Pectins}, url = {http://linkinghub.elsevier.com/retrieve/pii/S0960982208015340}, volume = {18}, year = {2008} }

TY - JOUR ID - DOI - 10.1016/j.cub.2008.10.065 L3 - citeulike-article-id:3822231 N2 - Plant organs are produced from meristems in a characteristic pattern. This pattern, referred to as phyllotaxis, is thought to be generated by local gradients of an information molecule, auxin [16]. Some studies propose a key role for the mechanical properties of the cell walls in the control of organ outgrowth [712]. A major cell-wall component is the linear ±-1-4-linked D-GalAp pectic polysaccharide homogalacturonan (HG), which plays a key role in cell-to-cell cohesion [13, 14]. HG is deposited in the cell wall in a highly (70%80%) methyl-esterified form and is subsequently de-methyl-esterified by pectin methyl-esterases (PME, EC 3.1.1.11). PME activity is itself regulated by endogenous PME inhibitor (PMEI) proteins [15]. PME action modulates cell-wall-matrix properties and plays a role in the control of cell growth [1618]. Here, we show that the formation of flower primordia in the Arabidopsis shoot apical meristem is accompanied by the de-methyl-esterification of pectic polysaccharides in the cell walls. In addition, experimental perturbation of the methyl-esterification status of pectins within the meristem dramatically alters the phyllotactic pattern. These results demonstrate that regulated de-methyl-esterification of pectins is a key event in the outgrowth of primordia and possibly also in phyllotactic patterning. IS - 24 SN - 0960-9822 EP - 1948 TI - Arabidopsis Phyllotaxis Is Controlled by the Methyl-Esterification Status of Cell-Wall Pectins VL - 18 SP - 1943 KW - cellbio KW - devbio KW - phyllotaxis AU - Peaucelle, Alexis AU - Louvet, Romain AU - Johansen, Jorunn AU - Höfte, Herman AU - Laufs, Patrick AU - Pelloux, Jérome AU - Mouille, Grégory PY - 2008/12/23/ UR - http://linkinghub.elsevier.com/retrieve/pii/S0960982208015340 ER -