Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis Export

@article{citeulike:5908412, abstract = {10.1073/pnas.0907095106 Abscisic acid (ABA) signaling is important for stress responses and developmental processes in plants. A subgroup of protein phosphatase 2C (group A PP2C) or SNF1-related protein kinase 2 (subclass III SnRK2) have been known as major negative or positive regulators of ABA signaling, respectively. Here, we demonstrate the physical and functional linkage between these two major signaling factors. Group A PP2Cs interacted physically with SnRK2s in various combinations, and efficiently inactivated ABA-activated SnRK2s via dephosphorylation of multiple Ser/Thr residues in the activation loop. This step was suppressed by the RCAR/PYR ABA receptors in response to ABA. However the abi1\^{a}€“1 mutated PP2C did not respond to the receptors and constitutively inactivated SnRK2. Our results demonstrate that group A PP2Cs act as \^{a}€˜gatekeepers\^{a}€™ of subclass III SnRK2s, unraveling an important regulatory mechanism of ABA signaling.}, author = {Umezawa, Taishi and Sugiyama, Naoyuki and Mizoguchi, Masahide and Hayashi, Shimpei and Myouga, Fumiyoshi and Yamaguchi-Shinozaki, Kazuko and Ishihama, Yasushi and Hirayama, Takashi and Shinozaki, Kazuo}, citeulike-article-id = {5908412}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0907095106}, citeulike-linkout-1 = {http://www.pnas.org/content/106/41/17588.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/106/41/17588.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19805022}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19805022}, day = {13}, doi = {10.1073/pnas.0907095106}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences}, keywords = {abscisic-acid, signaling}, month = {October}, number = {41}, pages = {17588--17593}, posted-at = {2009-11-13 12:11:51}, priority = {2}, title = {Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis}, url = {http://dx.doi.org/10.1073/pnas.0907095106}, volume = {106}, year = {2009} }

TY - JOUR ID - citeulike:5908412 L3 - citeulike-article-id:5908412 N2 - 10.1073/pnas.0907095106 Abscisic acid (ABA) signaling is important for stress responses and developmental processes in plants. A subgroup of protein phosphatase 2C (group A PP2C) or SNF1-related protein kinase 2 (subclass III SnRK2) have been known as major negative or positive regulators of ABA signaling, respectively. Here, we demonstrate the physical and functional linkage between these two major signaling factors. Group A PP2Cs interacted physically with SnRK2s in various combinations, and efficiently inactivated ABA-activated SnRK2s via dephosphorylation of multiple Ser/Thr residues in the activation loop. This step was suppressed by the RCAR/PYR ABA receptors in response to ABA. However the abi1–1 mutated PP2C did not respond to the receptors and constitutively inactivated SnRK2. Our results demonstrate that group A PP2Cs act as ‘gatekeepers’ of subclass III SnRK2s, unraveling an important regulatory mechanism of ABA signaling. IS - 41 JF - Proceedings of the National Academy of Sciences SN - 1091-6490 EP - 17593 TI - Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis VL - 106 SP - 17588 KW - abscisic-acid KW - signaling AU - Umezawa, Taishi AU - Sugiyama, Naoyuki AU - Mizoguchi, Masahide AU - Hayashi, Shimpei AU - Myouga, Fumiyoshi AU - Yamaguchi-Shinozaki, Kazuko AU - Ishihama, Yasushi AU - Hirayama, Takashi AU - Shinozaki, Kazuo PY - 2009/10/13/ UR - http://dx.doi.org/10.1073/pnas.0907095106 ER -