Effects of Biological Reactions and Modifications on Conductance of Nanofluidic Channels Export

@article{citeulike:6043992, abstract = {PMID: 16159198 Conductance characteristics of nanofluidic channels (nanochannels) fall into two regimes: at low ionic concentrations, conductance is governed by surface charge while at high ionic concentrations it is determined by nanochannel geometry and bulk ionic concentration. We used aminosilane chemistry and streptavidinbiotin binding to study the effects of surface reactions on nanochannel conductance at different ionic concentrations. Immobilization of small molecules such as aminosilane or biotin mainly changes surface charge, affecting conductance only in the low concentration regime. However, streptavidin not only modifies surface charge but also occludes part of the channel, resulting in observable conductance changes in both regimes. Our observations reflect the interplay between the competing effects of charge and size of streptavidin on nanochannel conductance.}, author = {Karnik, Rohit and Castelino, Kenneth and Fan, Rong and Yang, Peidong and Majumdar, Arun}, citeulike-article-id = {6043992}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/nl050966e}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/nl050966e}, comment = {Influence of surface charge on nanochannel conductance. Channel functionalization by APTES - sulfo-NHS-SS-biotin - streptavidin.}, day = {1}, doi = {10.1021/nl050966e}, journal = {Nano Letters}, keywords = {biotin, channel, charge, conductance, functionalization, immobilization, nanochannel, streptavidin, surface}, month = {September}, number = {9}, pages = {1638--1642}, posted-at = {2009-10-30 19:06:57}, priority = {3}, title = {Effects of Biological Reactions and Modifications on Conductance of Nanofluidic Channels}, url = {http://dx.doi.org/10.1021/nl050966e}, volume = {5}, year = {2005} }

TY - JOUR ID - citeulike:6043992 L3 - citeulike-article-id:6043992 N2 - PMID: 16159198 Conductance characteristics of nanofluidic channels (nanochannels) fall into two regimes: at low ionic concentrations, conductance is governed by surface charge while at high ionic concentrations it is determined by nanochannel geometry and bulk ionic concentration. We used aminosilane chemistry and streptavidinbiotin binding to study the effects of surface reactions on nanochannel conductance at different ionic concentrations. Immobilization of small molecules such as aminosilane or biotin mainly changes surface charge, affecting conductance only in the low concentration regime. However, streptavidin not only modifies surface charge but also occludes part of the channel, resulting in observable conductance changes in both regimes. Our observations reflect the interplay between the competing effects of charge and size of streptavidin on nanochannel conductance. IS - 9 JF - Nano Letters EP - 1642 TI - Effects of Biological Reactions and Modifications on Conductance of Nanofluidic Channels VL - 5 SP - 1638 KW - biotin KW - channel KW - charge KW - conductance KW - functionalization KW - immobilization KW - nanochannel KW - streptavidin KW - surface N1 - Influence of surface charge on nanochannel conductance. Channel functionalization by APTES - sulfo-NHS-SS-biotin - streptavidin. AU - Karnik, Rohit AU - Castelino, Kenneth AU - Fan, Rong AU - Yang, Peidong AU - Majumdar, Arun PY - 2005/09/01/ UR - http://dx.doi.org/10.1021/nl050966e ER -