Polycomb-mediated methylation on Lys27 of histone H3 pre-marks genes for de novo methylation in cancer. Export

@article{citeulike:1049270, abstract = {Many genes associated with CpG islands undergo de novo methylation in cancer. Studies have suggested that the pattern of this modification may be partially determined by an instructive mechanism that recognizes specifically marked regions of the genome. Using chromatin immunoprecipitation analysis, here we show that genes methylated in cancer cells are specifically packaged with nucleosomes containing histone H3 trimethylated on Lys27. This chromatin mark is established on these unmethylated CpG island genes early in development and then maintained in differentiated cell types by the presence of an EZH2-containing Polycomb complex. In cancer cells, as opposed to normal cells, the presence of this complex brings about the recruitment of DNA methyl transferases, leading to de novo methylation. These results suggest that tumor-specific targeting of de novo methylation is pre-programmed by an established epigenetic system that normally has a role in marking embryonic genes for repression.}, address = {Department of Cellular Biochemistry and Human Genetics, The Hebrew University–Hadassah Medical School, Jerusalem 91120, Israel.}, author = {Schlesinger, Yeshayahu and Straussman, Ravid and Keshet, Ilana and Farkash, Shlomit and Hecht, Merav and Zimmerman, Joseph and Eden, Eran and Yakhini, Zohar and Ben-Shushan, Etti and Reubinoff, Benjamin E E. and Bergman, Yehudit and Simon, Itamar and Cedar, Howard}, citeulike-article-id = {1049270}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ng1950}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/ng1950}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17200670}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17200670}, day = {31}, doi = {10.1038/ng1950}, issn = {1061-4036}, journal = {Nat Genet}, keywords = {cancer, methylation, polycomb}, month = {December}, posted-at = {2009-05-11 18:33:29}, priority = {2}, title = {Polycomb-mediated methylation on Lys27 of histone H3 pre-marks genes for de novo methylation in cancer.}, url = {http://dx.doi.org/10.1038/ng1950}, year = {2006} }

TY - JOUR ID - citeulike:1049270 L3 - citeulike-article-id:1049270 N2 - Many genes associated with CpG islands undergo de novo methylation in cancer. Studies have suggested that the pattern of this modification may be partially determined by an instructive mechanism that recognizes specifically marked regions of the genome. Using chromatin immunoprecipitation analysis, here we show that genes methylated in cancer cells are specifically packaged with nucleosomes containing histone H3 trimethylated on Lys27. This chromatin mark is established on these unmethylated CpG island genes early in development and then maintained in differentiated cell types by the presence of an EZH2-containing Polycomb complex. In cancer cells, as opposed to normal cells, the presence of this complex brings about the recruitment of DNA methyl transferases, leading to de novo methylation. These results suggest that tumor-specific targeting of de novo methylation is pre-programmed by an established epigenetic system that normally has a role in marking embryonic genes for repression. JF - Nat Genet SN - 1061-4036 AD - Department of Cellular Biochemistry and Human Genetics, The Hebrew University–Hadassah Medical School, Jerusalem 91120, Israel. TI - Polycomb-mediated methylation on Lys27 of histone H3 pre-marks genes for de novo methylation in cancer. KW - cancer KW - methylation KW - polycomb AU - Schlesinger, Yeshayahu AU - Straussman, Ravid AU - Keshet, Ilana AU - Farkash, Shlomit AU - Hecht, Merav AU - Zimmerman, Joseph AU - Eden, Eran AU - Yakhini, Zohar AU - Ben-Shushan, Etti AU - Reubinoff, Benjamin E AU - Bergman, Yehudit AU - Simon, Itamar AU - Cedar, Howard PY - 2006/12/31/ UR - http://dx.doi.org/10.1038/ng1950 ER -