Whole-Genome Mapping of Histone H3 Lys4 and 27 Trimethylations Reveals Distinct Genomic Compartments in Human Embryonic Stem Cells Export

@article{citeulike:3666539, abstract = {Epigenetic modifications are crucial for proper lineage specification and embryo development. To explore the chromatin modification landscapes in human ES cells, we profiled two histone modifications, H3K4me3 and H3K27me3, by ChIP coupled with the paired-end ditags sequencing strategy. H3K4me3 was found to be a prevalent mark and occurred in close proximity to the promoters of two-thirds of total human genes. Among the H3K27me3 loci identified, 56\% are associated with promoters and the vast majority of them are comodified by H3K4me3. By deep-transcript digital counting, 80\% of H3K4me3 and 36\% of comodified promoters were found to be transcribed. Remarkably, we observed that different combinations of histone methylations are associated with genes from distinct functional categories. These global histone methylation maps provide an epigenetic framework that enables the discovery of novel transcriptional networks and delineation of different genetic compartments of the pluripotent cell genome.}, author = {Zhao, X. and Han, X. and Chew, J. and Liu, J. and Chiu, K. and Choo, A. and Orlov, Y. and Sung, W. and Shahab, A. and Kuznetsov, V.}, citeulike-article-id = {3666539}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.stem.2007.08.004}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1934590907001233}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18371363}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18371363}, day = {13}, doi = {10.1016/j.stem.2007.08.004}, issn = {19345909}, journal = {Cell Stem Cell}, keywords = {bivalent, chromatin-organization, h3k4, hek27}, month = {September}, number = {3}, pages = {286--298}, posted-at = {2009-03-23 19:54:02}, priority = {5}, title = {Whole-Genome Mapping of Histone H3 Lys4 and 27 Trimethylations Reveals Distinct Genomic Compartments in Human Embryonic Stem Cells}, url = {http://dx.doi.org/10.1016/j.stem.2007.08.004}, volume = {1}, year = {2007} }

TY - JOUR ID - citeulike:3666539 L3 - citeulike-article-id:3666539 N2 - Epigenetic modifications are crucial for proper lineage specification and embryo development. To explore the chromatin modification landscapes in human ES cells, we profiled two histone modifications, H3K4me3 and H3K27me3, by ChIP coupled with the paired-end ditags sequencing strategy. H3K4me3 was found to be a prevalent mark and occurred in close proximity to the promoters of two-thirds of total human genes. Among the H3K27me3 loci identified, 56% are associated with promoters and the vast majority of them are comodified by H3K4me3. By deep-transcript digital counting, 80% of H3K4me3 and 36% of comodified promoters were found to be transcribed. Remarkably, we observed that different combinations of histone methylations are associated with genes from distinct functional categories. These global histone methylation maps provide an epigenetic framework that enables the discovery of novel transcriptional networks and delineation of different genetic compartments of the pluripotent cell genome. IS - 3 JF - Cell Stem Cell SN - 19345909 EP - 298 TI - Whole-Genome Mapping of Histone H3 Lys4 and 27 Trimethylations Reveals Distinct Genomic Compartments in Human Embryonic Stem Cells VL - 1 SP - 286 KW - bivalent KW - chromatin-organization KW - h3k4 KW - hek27 AU - Zhao, X AU - Han, X AU - Chew, J AU - Liu, J AU - Chiu, K AU - Choo, A AU - Orlov, Y AU - Sung, W AU - Shahab, A AU - Kuznetsov, V PY - 2007/09/13/ UR - http://dx.doi.org/10.1016/j.stem.2007.08.004 ER -