Methylation of H3 K4 and K79 is not strictly dependent on H2B K123 ubiquitylation Export

@article{citeulike:4144029, abstract = {Covalent modifications of histone proteins have profound consequences on chromatin structure and function. Specific modification patterns constitute a code read by effector proteins. Studies from yeast found that H3 trimethylation at K4 and K79 is dependent on ubiquitylation of H2B K123, which is termed a "trans-tail pathway." In this study, we show that a strain unable to be ubiquitylated on H2B (K123R) is still proficient for H3 trimethylation at both K4 and K79, indicating that H3 methylation status is not solely dependent on H2B ubiquitylation. However, additional mutations in H2B result in loss of H3 methylation when combined with htb1-K123R. Consistent with this, we find that the original strain used to identify the trans-tail pathway has a genomic mutation that, when combined with H2B K123R, results in defective H3 methylation. Finally, we show that strains lacking the ubiquitin ligase Bre1 are defective for H3 methylation, suggesting that there is an additional Bre1 substrate that in combination with H2B K123 facilitates H3 methylation. 10.1083/jcb.200812088}, author = {Foster, Elinor R. and Downs, Jessica A.}, citeulike-article-id = {4144029}, citeulike-linkout-0 = {http://dx.doi.org/10.1083/jcb.200812088}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19255247}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19255247}, day = {2}, doi = {10.1083/jcb.200812088}, journal = {J. Cell Biol.}, keywords = {h3k4, h3k79, ubiquitylation}, month = {March}, pages = {jcb.200812088+}, posted-at = {2009-03-06 14:59:14}, priority = {2}, title = {Methylation of H3 K4 and K79 is not strictly dependent on H2B K123 ubiquitylation}, url = {http://dx.doi.org/10.1083/jcb.200812088}, year = {2009} }

TY - JOUR ID - citeulike:4144029 L3 - citeulike-article-id:4144029 N2 - Covalent modifications of histone proteins have profound consequences on chromatin structure and function. Specific modification patterns constitute a code read by effector proteins. Studies from yeast found that H3 trimethylation at K4 and K79 is dependent on ubiquitylation of H2B K123, which is termed a "trans-tail pathway." In this study, we show that a strain unable to be ubiquitylated on H2B (K123R) is still proficient for H3 trimethylation at both K4 and K79, indicating that H3 methylation status is not solely dependent on H2B ubiquitylation. However, additional mutations in H2B result in loss of H3 methylation when combined with htb1-K123R. Consistent with this, we find that the original strain used to identify the trans-tail pathway has a genomic mutation that, when combined with H2B K123R, results in defective H3 methylation. Finally, we show that strains lacking the ubiquitin ligase Bre1 are defective for H3 methylation, suggesting that there is an additional Bre1 substrate that in combination with H2B K123 facilitates H3 methylation. 10.1083/jcb.200812088 JF - J. Cell Biol. TI - Methylation of H3 K4 and K79 is not strictly dependent on H2B K123 ubiquitylation SP - jcb.200812088 KW - h3k4 KW - h3k79 KW - ubiquitylation AU - Foster, Elinor AU - Downs, Jessica PY - 2009/03/02/ UR - http://dx.doi.org/10.1083/jcb.200812088 ER -