CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3|[prime]|-end processing Export

@article{citeulike:5054233, abstract = {Post-translational histone modifications have essential roles in controlling nuclear processes; however, the specific mechanisms regulating these modifications and their combinatorial activities remain elusive. Cyclin-dependent kinase 9 (CDK9) regulates gene expression by phosphorylating transcriptional regulatory proteins, including the RNA polymerase II carboxy-terminal domain. Here, we show that CDK9 activity is essential for maintaining global and gene-associated levels of histone H2B monoubiquitination (H2Bub1). Furthermore, CDK9 activity and H2Bub1 help to maintain correct replication-dependent histone messenger RNA (mRNA) 3'-end processing. CDK9 knockdown consistently resulted in inefficient recognition of the correct mRNA 3'-end cleavage site and led to increased read-through of RNA polymerase II to an alternative downstream polyadenylation signal. Thus, CDK9 acts to integrate phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing.}, author = {Pirngruber, Judith and Shchebet, Andrei and Schreiber, Lisa and Shema, Efrat and Minsky, Neri and Chapman, Rob D. and Eick, Dirk and Aylon, Yael and Oren, Moshe and Johnsen, Steven A.}, citeulike-article-id = {5054233}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/embor.2009.108}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/embor2009108}, day = {03}, doi = {10.1038/embor.2009.108}, issn = {1469-221X}, journal = {EMBO reports}, keywords = {ctd, d-polymerase}, month = {July}, number = {8}, pages = {894--900}, posted-at = {2009-11-08 16:40:33}, priority = {2}, publisher = {Nature Publishing Group}, title = {CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3|[prime]|-end processing}, url = {http://dx.doi.org/10.1038/embor.2009.108}, volume = {10}, year = {2009} }

TY - JOUR ID - citeulike:5054233 L3 - citeulike-article-id:5054233 N2 - Post-translational histone modifications have essential roles in controlling nuclear processes; however, the specific mechanisms regulating these modifications and their combinatorial activities remain elusive. Cyclin-dependent kinase 9 (CDK9) regulates gene expression by phosphorylating transcriptional regulatory proteins, including the RNA polymerase II carboxy-terminal domain. Here, we show that CDK9 activity is essential for maintaining global and gene-associated levels of histone H2B monoubiquitination (H2Bub1). Furthermore, CDK9 activity and H2Bub1 help to maintain correct replication-dependent histone messenger RNA (mRNA) 3'-end processing. CDK9 knockdown consistently resulted in inefficient recognition of the correct mRNA 3'-end cleavage site and led to increased read-through of RNA polymerase II to an alternative downstream polyadenylation signal. Thus, CDK9 acts to integrate phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. IS - 8 JF - EMBO reports SN - 1469-221X EP - 900 TI - CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3|[prime]|-end processing PB - Nature Publishing Group VL - 10 SP - 894 KW - ctd KW - d-polymerase AU - Pirngruber, Judith AU - Shchebet, Andrei AU - Schreiber, Lisa AU - Shema, Efrat AU - Minsky, Neri AU - Chapman, Rob AU - Eick, Dirk AU - Aylon, Yael AU - Oren, Moshe AU - Johnsen, Steven PY - 2009/07/03/ UR - http://dx.doi.org/10.1038/embor.2009.108 ER -