Trans-acting antisense RNAs mediate transcriptional gene cosuppression in S. cerevisiae. Export

@article{citeulike:5084726, abstract = {Homology-dependent gene silencing, a phenomenon described as cosuppression in plants, depends on siRNAs. We provide evidence that in Saccharomyces cerevisiae, which is missing the RNAi machinery, protein coding gene cosuppression exists. Indeed, introduction of an additional copy of PHO84 on a plasmid or within the genome results in the cosilencing of both the transgene and the endogenous gene. This repression is transcriptional and position-independent and requires trans-acting antisense RNAs. Antisense RNAs induce transcriptional gene silencing both in cis and in trans, and the two pathways differ by the implication of the Hda1/2/3 complex. We also show that trans-silencing is influenced by the Set1 histone methyltransferase, which promotes antisense RNA production. Finally we show that although antisense-mediated cis-silencing occurs in other genes, trans-silencing so far depends on features specific to PHO84. All together our data highlight the importance of noncoding RNAs in mediating RNAi-independent transcriptional gene silencing.}, author = {Camblong, Jurgi and Beyrouthy, Nissrine and Guffanti, Elisa and Schlaepfer, Guillaume and Steinmetz, Lars M. and Stutz, Fran\c{c}oise}, citeulike-article-id = {5084726}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gad.522509}, citeulike-linkout-1 = {http://genesdev.cshlp.org/content/23/13/1534.abstract}, citeulike-linkout-2 = {http://genesdev.cshlp.org/content/23/13/1534.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19571181}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19571181}, day = {1}, doi = {10.1101/gad.522509}, issn = {1549-5477}, journal = {Genes \& development}, keywords = {antisense}, month = {July}, number = {13}, pages = {1534--1545}, posted-at = {2009-08-05 16:59:18}, priority = {2}, title = {Trans-acting antisense RNAs mediate transcriptional gene cosuppression in S. cerevisiae.}, url = {http://dx.doi.org/10.1101/gad.522509}, volume = {23}, year = {2009} }

TY - JOUR ID - citeulike:5084726 L3 - citeulike-article-id:5084726 N2 - Homology-dependent gene silencing, a phenomenon described as cosuppression in plants, depends on siRNAs. We provide evidence that in Saccharomyces cerevisiae, which is missing the RNAi machinery, protein coding gene cosuppression exists. Indeed, introduction of an additional copy of PHO84 on a plasmid or within the genome results in the cosilencing of both the transgene and the endogenous gene. This repression is transcriptional and position-independent and requires trans-acting antisense RNAs. Antisense RNAs induce transcriptional gene silencing both in cis and in trans, and the two pathways differ by the implication of the Hda1/2/3 complex. We also show that trans-silencing is influenced by the Set1 histone methyltransferase, which promotes antisense RNA production. Finally we show that although antisense-mediated cis-silencing occurs in other genes, trans-silencing so far depends on features specific to PHO84. All together our data highlight the importance of noncoding RNAs in mediating RNAi-independent transcriptional gene silencing. IS - 13 JF - Genes & development SN - 1549-5477 EP - 1545 TI - Trans-acting antisense RNAs mediate transcriptional gene cosuppression in S. cerevisiae. VL - 23 SP - 1534 KW - antisense AU - Camblong, Jurgi AU - Beyrouthy, Nissrine AU - Guffanti, Elisa AU - Schlaepfer, Guillaume AU - Steinmetz, Lars AU - Stutz, Françoise PY - 2009/07/01/ UR - http://dx.doi.org/10.1101/gad.522509 ER -