Nuclear and division-plane positioning revealed by optical micromanipulation. Export

@article{citeulike:2638417, abstract = {The position of the division plane affects cell shape and size, as well as tissue organization. Cells of the fission yeast Schizosaccharomyces pombe have a centrally placed nucleus and divide by fission at the cell center. Microtubules (MTs) are required for the central position of the nucleus. Genetic studies lead to the hypothesis that the position of the nucleus may determine the position of the division plane. Alternatively, the division plane may be positioned by the spindle or by morphogen gradients or reaction diffusion mechanisms. Here, we investigate the role of MTs in nuclear positioning and the role of the nucleus in division-plane positioning by displacing the nucleus with optical tweezers. A displaced nucleus returned to the cell center by MT pushing against the cell tips. Nuclear displacement during interphase or early prophase resulted in asymmetric cell division, whereas displacement during prometaphase resulted in symmetric division as in unmanipulated cells. These results suggest that the division plane is specified by the predividing nucleus. Because the yeast nucleus is centered by MTs during interphase but not in mitosis, we hypothesize that the establishment of the division plane at the beginning of mitosis is an optimal mechanism for accurate symmetric division in these cells.}, address = {European Laboratory for Nonlinear Spectroscopy, Via Nello Carrara 1, 50019 Sesto Fiorentino (Florence), Italy. tolic@mpi-cbg.de}, author = {Tolic-N{\o}rrelykke, I. M. and Sacconi, L. and Stringari, C. and Raabe, I. and Pavone, F. S.}, citeulike-article-id = {2638417}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cub.2005.05.052}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16005294}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16005294}, day = {12}, doi = {10.1016/j.cub.2005.05.052}, issn = {0960-9822}, journal = {Current biology : CB}, month = {July}, number = {13}, pages = {1212--1216}, posted-at = {2008-04-13 18:28:38}, priority = {2}, title = {Nuclear and division-plane positioning revealed by optical micromanipulation.}, url = {http://dx.doi.org/10.1016/j.cub.2005.05.052}, volume = {15}, year = {2005} }

TY - JOUR ID - citeulike:2638417 L3 - citeulike-article-id:2638417 N2 - The position of the division plane affects cell shape and size, as well as tissue organization. Cells of the fission yeast Schizosaccharomyces pombe have a centrally placed nucleus and divide by fission at the cell center. Microtubules (MTs) are required for the central position of the nucleus. Genetic studies lead to the hypothesis that the position of the nucleus may determine the position of the division plane. Alternatively, the division plane may be positioned by the spindle or by morphogen gradients or reaction diffusion mechanisms. Here, we investigate the role of MTs in nuclear positioning and the role of the nucleus in division-plane positioning by displacing the nucleus with optical tweezers. A displaced nucleus returned to the cell center by MT pushing against the cell tips. Nuclear displacement during interphase or early prophase resulted in asymmetric cell division, whereas displacement during prometaphase resulted in symmetric division as in unmanipulated cells. These results suggest that the division plane is specified by the predividing nucleus. Because the yeast nucleus is centered by MTs during interphase but not in mitosis, we hypothesize that the establishment of the division plane at the beginning of mitosis is an optimal mechanism for accurate symmetric division in these cells. IS - 13 JF - Current biology : CB SN - 0960-9822 AD - European Laboratory for Nonlinear Spectroscopy, Via Nello Carrara 1, 50019 Sesto Fiorentino (Florence), Italy. tolic@mpi-cbg.de EP - 1216 TI - Nuclear and division-plane positioning revealed by optical micromanipulation. VL - 15 SP - 1212 AU - Tolic-Nørrelykke, IM AU - Sacconi, L AU - Stringari, C AU - Raabe, I AU - Pavone, FS PY - 2005/07/12/ UR - http://dx.doi.org/10.1016/j.cub.2005.05.052 ER -