Cytokinetic furrowing in toroidal, binucleate and anucleate cells in C. elegans embryos. Export

@article{citeulike:2648784, abstract = {Classical experimental studies on echinoderm zygotes concluded that the juxtaposition of two astral microtubule arrays localizes the stimulus for cytokinetic furrowing. However, recent experimental and genetic studies in Caenorhabditis elegans, Drosophila and mammalian cultured cells implicate microtubules of the central spindle, and regulatory proteins associated with this structure, suggesting that the essential conditions for furrow induction may differ from one animal cell to another. We used micromanipulation and laser microsurgery to create, in three ways, the juxtaposition of astral microtubules in C. elegans embryonic cells. In toroidal cells we observe that furrows initiate both where astral microtubule arrays are juxtaposed, and where the cortex most closely approaches the central spindle. We find that binucleate cells successfully furrow not only across the spindles, but also between unconnected spindle poles. Finally, we find that anucleate cells containing only a pair of centrosomes nevertheless attempt to cleave. Therefore, in C. elegans embryonic cells, as in echinoderms, juxtaposition of two asters suffices to induce furrowing, and neither the chromatin nor the physical structure of the central spindle are indispensable for furrow initiation. However, furrows that cross a central spindle are more likely to complete than those that do not.}, address = {Center for Cell Dynamics, Friday Harbor Laboratories, 620 University Road, Friday Harbor, WA 98250, USA.}, author = {Baruni, J. K. and Munro, E. M. and von Dassow, G.}, citeulike-article-id = {2648784}, citeulike-linkout-0 = {http://dx.doi.org/10.1242/jcs.022897}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18198185}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18198185}, citeulike-linkout-3 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031431}, day = {1}, doi = {10.1242/jcs.022897}, issn = {0021-9533}, journal = {Journal of cell science}, keywords = {anucleate, cell}, month = {February}, number = {Pt 3}, pages = {306--316}, posted-at = {2008-04-10 10:48:22}, priority = {2}, title = {Cytokinetic furrowing in toroidal, binucleate and anucleate cells in C. elegans embryos.}, url = {http://dx.doi.org/10.1242/jcs.022897}, volume = {121}, year = {2008} }

TY - JOUR ID - citeulike:2648784 L3 - citeulike-article-id:2648784 N2 - Classical experimental studies on echinoderm zygotes concluded that the juxtaposition of two astral microtubule arrays localizes the stimulus for cytokinetic furrowing. However, recent experimental and genetic studies in Caenorhabditis elegans, Drosophila and mammalian cultured cells implicate microtubules of the central spindle, and regulatory proteins associated with this structure, suggesting that the essential conditions for furrow induction may differ from one animal cell to another. We used micromanipulation and laser microsurgery to create, in three ways, the juxtaposition of astral microtubules in C. elegans embryonic cells. In toroidal cells we observe that furrows initiate both where astral microtubule arrays are juxtaposed, and where the cortex most closely approaches the central spindle. We find that binucleate cells successfully furrow not only across the spindles, but also between unconnected spindle poles. Finally, we find that anucleate cells containing only a pair of centrosomes nevertheless attempt to cleave. Therefore, in C. elegans embryonic cells, as in echinoderms, juxtaposition of two asters suffices to induce furrowing, and neither the chromatin nor the physical structure of the central spindle are indispensable for furrow initiation. However, furrows that cross a central spindle are more likely to complete than those that do not. IS - Pt 3 JF - Journal of cell science SN - 0021-9533 AD - Center for Cell Dynamics, Friday Harbor Laboratories, 620 University Road, Friday Harbor, WA 98250, USA. EP - 316 TI - Cytokinetic furrowing in toroidal, binucleate and anucleate cells in C. elegans embryos. VL - 121 SP - 306 KW - anucleate KW - cell AU - Baruni, JK AU - Munro, EM AU - von Dassow, G PY - 2008/02/01/ UR - http://dx.doi.org/10.1242/jcs.022897 ER -