A loop-mediated isothermal amplification method targets the phoP gene for the detection of Salmonella in food samples Export

@article{citeulike:4739719, abstract = {A loop-mediated isothermal amplification (LAMP) assay was developed and validated for the specific detection of Salmonella in food. The assay used specifically designed primers to target within the phoP gene and correctly identified all 66 strains of Salmonella and 73 non- Salmonella strains tested. The phoP gene regulates the expression of genes involved in virulence and the survival of Salmonella from destruction by macrophage. The probability of detection was 100\% when a Salmonella cell suspension containing 10 1  CFU/ml was used as a template in the LAMP assay. Prior to the LAMP assay, a sample preparation protocol was applied that included a pre-enrichment step in buffered peptone water, followed by extraction and purification of DNA. In this way, 85 various food samples were investigated for Salmonella including minced meat of pig and raw milk. The diagnostic accuracy was shown to be 100\% when compared to the traditional culture method. This combination of sample enrichment, and LAMP assay can detect 35 CFU per 250 ml of prepared food samples. The overall analysis time for the LAMP assay method was approximately 24 h. This is in contrast to 5 to 7 days of analysis time required for the traditional culture method. Consequently, the LAMP described here has the potential to become a standardized method for the rapid detection of Salmonella in diagnostic laboratories once further validated by inter-laboratory studies.}, author = {Li, Xuefei and Zhang, Shu and Zhang, Hongwei and Zhang, Lihuai and Tao, Haitao and Yu, Jia and Zheng, Wenjie and Liu, Chenghu and L\"{u}, Dan and Xiang, Rong}, citeulike-article-id = {4739719}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ijfoodmicro.2009.05.027}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0168160509002967}, day = {15}, doi = {10.1016/j.ijfoodmicro.2009.05.027}, issn = {01681605}, journal = {International Journal of Food Microbiology}, keywords = {food-safety, loop-mediated-isothermal-amplification, salmonella}, month = {August}, number = {3}, pages = {252--258}, posted-at = {2009-07-20 20:04:20}, priority = {2}, title = {A loop-mediated isothermal amplification method targets the phoP gene for the detection of Salmonella in food samples}, url = {http://dx.doi.org/10.1016/j.ijfoodmicro.2009.05.027}, volume = {133}, year = {2009} }

TY - JOUR ID - citeulike:4739719 L3 - citeulike-article-id:4739719 N2 - A loop-mediated isothermal amplification (LAMP) assay was developed and validated for the specific detection of Salmonella in food. The assay used specifically designed primers to target within the phoP gene and correctly identified all 66 strains of Salmonella and 73 non- Salmonella strains tested. The phoP gene regulates the expression of genes involved in virulence and the survival of Salmonella from destruction by macrophage. The probability of detection was 100% when a Salmonella cell suspension containing 10 1  CFU/ml was used as a template in the LAMP assay. Prior to the LAMP assay, a sample preparation protocol was applied that included a pre-enrichment step in buffered peptone water, followed by extraction and purification of DNA. In this way, 85 various food samples were investigated for Salmonella including minced meat of pig and raw milk. The diagnostic accuracy was shown to be 100% when compared to the traditional culture method. This combination of sample enrichment, and LAMP assay can detect 35 CFU per 250 ml of prepared food samples. The overall analysis time for the LAMP assay method was approximately 24 h. This is in contrast to 5 to 7 days of analysis time required for the traditional culture method. Consequently, the LAMP described here has the potential to become a standardized method for the rapid detection of Salmonella in diagnostic laboratories once further validated by inter-laboratory studies. IS - 3 JF - International Journal of Food Microbiology SN - 01681605 EP - 258 TI - A loop-mediated isothermal amplification method targets the phoP gene for the detection of Salmonella in food samples VL - 133 SP - 252 KW - food-safety KW - loop-mediated-isothermal-amplification KW - salmonella AU - Li, Xuefei AU - Zhang, Shu AU - Zhang, Hongwei AU - Zhang, Lihuai AU - Tao, Haitao AU - Yu, Jia AU - Zheng, Wenjie AU - Liu, Chenghu AU - Lü, Dan AU - Xiang, Rong PY - 2009/08/15/ UR - http://dx.doi.org/10.1016/j.ijfoodmicro.2009.05.027 ER -