Electroformation of Giant Vesicles from an Inverse Phase Precursor Export

@article{citeulike:4350031, abstract = {We discuss a simple modification of the well-known method of giant vesicle electroformation that allows for a direct addition of water-soluble species to the phospholipid bilayers. Using this modified method, we prepare phospholipid vesicles decorated with chitosan, a water-soluble polysaccharide currently investigated for potential pharmacological applications. We find that the method allows this polysaccharide with primary amino groups on every glucose subunit to be tightly bound to the membrane, rather than simply being encapsulated.}, author = {Mertins, Omar and da Silveira, N\'{a}dya P. and Pohlmann, Adriana R. and Schr\"{o}der, Andr\'{e} P. and Marques, Carlos M.}, citeulike-article-id = {4350031}, citeulike-linkout-0 = {http://www.cell.com/biophysj/abstract/S0006-3495(09)00389-0}, day = {8}, keywords = {chitosan, dopc, electroformation, emulsion, fluorescent, methods}, month = {April}, number = {7}, pages = {2719--2726}, posted-at = {2009-04-17 20:52:57}, priority = {2}, title = {Electroformation of Giant Vesicles from an Inverse Phase Precursor}, url = {http://www.cell.com/biophysj/abstract/S0006-3495(09)00389-0}, volume = {96}, year = {2009} }

TY - JOUR ID - citeulike:4350031 L3 - citeulike-article-id:4350031 N2 - We discuss a simple modification of the well-known method of giant vesicle electroformation that allows for a direct addition of water-soluble species to the phospholipid bilayers. Using this modified method, we prepare phospholipid vesicles decorated with chitosan, a water-soluble polysaccharide currently investigated for potential pharmacological applications. We find that the method allows this polysaccharide with primary amino groups on every glucose subunit to be tightly bound to the membrane, rather than simply being encapsulated. IS - 7 EP - 2726 TI - Electroformation of Giant Vesicles from an Inverse Phase Precursor VL - 96 SP - 2719 KW - chitosan KW - dopc KW - electroformation KW - emulsion KW - fluorescent KW - methods AU - Mertins, Omar AU - da Silveira, Nádya AU - Pohlmann, Adriana AU - Schröder, André AU - Marques, Carlos PY - 2009/04/08/ UR - http://www.cell.com/biophysj/abstract/S0006-3495(09)00389-0 ER -