A functional genomic screen identifies a role for TAO1 kinase in spindle-checkpoint signalling. Export

@article{citeulike:1219587, abstract = {Defects in chromosome-microtubule attachment trigger spindle-checkpoint activation and delay mitotic progression. How microtubule attachment is sensed and integrated into the steps of checkpoint-signal amplification is poorly understood. In a functional genomic screen targeting human kinases and phosphatases, we identified a microtubule affinity-regulating kinase kinase, TAO1 (also known as MARKK) as an important regulator of mitotic progression, required for both chromosome congression and checkpoint-induced anaphase delay. TAO1 interacts with the checkpoint kinase BubR1 and promotes enrichment of the checkpoint protein Mad2 at sites of defective attachment, providing evidence for a regulatory step that precedes the proposed Mad2-Mad1 dependent checkpoint-signal amplification step. We propose that the dual functions of TAO1 in regulating microtubule dynamics and checkpoint signalling may help to coordinate the establishment and monitoring of correct congression of chromosomes, thereby protecting genomic stability in human cells.}, address = {[1] Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA and Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. [2] These authors contributed equally to this work.}, author = {Draviam, Viji M M. and Stegmeier, Frank and Nalepa, Grzegorz and Sowa, Mathew E E. and Chen, Jing and Liang, Anthony and Hannon, Gregory J J. and Sorger, Peter K K. and Harper, J Wade W. and Elledge, Stephen J J.}, citeulike-article-id = {1219587}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ncb1569}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17417629}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17417629}, day = {8}, doi = {10.1038/ncb1569}, issn = {1465-7392}, journal = {Nat Cell Biol}, keywords = {chromosome\_segregation}, month = {April}, posted-at = {2007-04-11 02:00:23}, priority = {2}, title = {A functional genomic screen identifies a role for TAO1 kinase in spindle-checkpoint signalling.}, url = {http://dx.doi.org/10.1038/ncb1569}, year = {2007} }

TY - JOUR ID - citeulike:1219587 L3 - citeulike-article-id:1219587 N2 - Defects in chromosome-microtubule attachment trigger spindle-checkpoint activation and delay mitotic progression. How microtubule attachment is sensed and integrated into the steps of checkpoint-signal amplification is poorly understood. In a functional genomic screen targeting human kinases and phosphatases, we identified a microtubule affinity-regulating kinase kinase, TAO1 (also known as MARKK) as an important regulator of mitotic progression, required for both chromosome congression and checkpoint-induced anaphase delay. TAO1 interacts with the checkpoint kinase BubR1 and promotes enrichment of the checkpoint protein Mad2 at sites of defective attachment, providing evidence for a regulatory step that precedes the proposed Mad2-Mad1 dependent checkpoint-signal amplification step. We propose that the dual functions of TAO1 in regulating microtubule dynamics and checkpoint signalling may help to coordinate the establishment and monitoring of correct congression of chromosomes, thereby protecting genomic stability in human cells. JF - Nat Cell Biol SN - 1465-7392 AD - [1] Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA and Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. [2] These authors contributed equally to this work. TI - A functional genomic screen identifies a role for TAO1 kinase in spindle-checkpoint signalling. KW - chromosome_segregation AU - Draviam, Viji M AU - Stegmeier, Frank AU - Nalepa, Grzegorz AU - Sowa, Mathew E AU - Chen, Jing AU - Liang, Anthony AU - Hannon, Gregory J AU - Sorger, Peter K AU - Harper, J Wade AU - Elledge, Stephen J PY - 2007/04/08/ UR - http://dx.doi.org/10.1038/ncb1569 ER -