Genome-Wide RNAi Screen Identifies Genes Involved in Intestinal Pathogenic Bacterial Infection Export

@article{citeulike:5381889, abstract = {Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. Therefore, we revealed multiple genes involved in antibacterial defense and the regulation of innate immunity. 10.1126/science.1173164}, author = {Cronin, Shane J. F. and Nehme, Nadine T. and Limmer, Stefanie and Liegeois, Samuel and Pospisilik, J. Andrew and Schramek, Daniel and Leibbrandt, Andreas and Simoes, Ricardo and Gruber, Susanne and Puc, Urszula and Ebersberger, Ingo and Zoranovic, Tamara and Neely, Gregory G. and von Haeseler, Arndt and Ferrandon, Dominique and Penninger, Josef M.}, citeulike-article-id = {5381889}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1173164}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/325/5938/340}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19520911}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19520911}, day = {17}, doi = {10.1126/science.1173164}, issn = {1095-9203}, journal = {Science}, keywords = {geneticmod, multiplexing, rnai, twostar}, month = {July}, number = {5938}, pages = {340--343}, posted-at = {2009-08-06 23:04:46}, priority = {2}, title = {Genome-Wide RNAi Screen Identifies Genes Involved in Intestinal Pathogenic Bacterial Infection}, url = {http://dx.doi.org/10.1126/science.1173164}, volume = {325}, year = {2009} }

TY - JOUR ID - citeulike:5381889 L3 - citeulike-article-id:5381889 N2 - Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. Therefore, we revealed multiple genes involved in antibacterial defense and the regulation of innate immunity. 10.1126/science.1173164 IS - 5938 JF - Science SN - 1095-9203 EP - 343 TI - Genome-Wide RNAi Screen Identifies Genes Involved in Intestinal Pathogenic Bacterial Infection VL - 325 SP - 340 KW - geneticmod KW - multiplexing KW - rnai KW - twostar AU - Cronin, Shane AU - Nehme, Nadine AU - Limmer, Stefanie AU - Liegeois, Samuel AU - Pospisilik, Andrew AU - Schramek, Daniel AU - Leibbrandt, Andreas AU - Simoes, Ricardo AU - Gruber, Susanne AU - Puc, Urszula AU - Ebersberger, Ingo AU - Zoranovic, Tamara AU - Neely, Gregory AU - von Haeseler, Arndt AU - Ferrandon, Dominique AU - Penninger, Josef PY - 2009/07/17/ UR - http://dx.doi.org/10.1126/science.1173164 ER -