A rapid and efficient single-cell manipulation method for screening antigen-specific antibody|[ndash]|secreting cells from human peripheral blood Export

@article{citeulike:5453897, abstract = {Antigen-specific human monoclonal antibodies (mAbs) are key candidates for therapeutic agents. However, the availability of a suitable screening system for antigen-specific antibody–secreting cells (ASCs) is limited in humans. Here we present a unique method for detecting individual ASCs using microwell array chips, which enables the analysis of live cells on a single-cell basis and offers a rapid, efficient and high-throughput (up to 234,000 individual cells) system for identifying and recovering objective ASCs. We applied the system to detect and retrieve ASCs for hepatitis B virus and influenza viruses from human peripheral blood lymphocytes and produced human mAbs with virus-neutralizing activities within a week. Furthermore, we show that the system is useful for detecting ASCs for multiple antigens as well as for selection of ASCs secreting high-affinity antibodies on a chip. Our method can open the way for the generation of therapeutic antibodies for individual patients.}, author = {Jin, Aishun and Ozawa, Tatsuhiko and Tajiri, Kazuto and Obata, Tsutomu and Kondo, Sachiko and Kinoshita, Koshi and Kadowaki, Shinichi and Takahashi, Kazuo and Sugiyama, Toshiro and Kishi, Hiroyuki and Muraguchi, Atsushi}, citeulike-article-id = {5453897}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nm.1966}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nm.1966}, day = {16}, doi = {10.1038/nm.1966}, issn = {1078-8956}, journal = {Nature Medicine}, keywords = {fourstar, immunology, method, microfluidics, multiplexing, screening}, month = {August}, number = {9}, pages = {1088--1092}, posted-at = {2009-09-05 01:26:56}, priority = {2}, publisher = {Nature Publishing Group}, title = {A rapid and efficient single-cell manipulation method for screening antigen-specific antibody|[ndash]|secreting cells from human peripheral blood}, url = {http://dx.doi.org/10.1038/nm.1966}, volume = {15}, year = {2009} }

TY - JOUR ID - citeulike:5453897 L3 - citeulike-article-id:5453897 N2 - Antigen-specific human monoclonal antibodies (mAbs) are key candidates for therapeutic agents. However, the availability of a suitable screening system for antigen-specific antibody–secreting cells (ASCs) is limited in humans. Here we present a unique method for detecting individual ASCs using microwell array chips, which enables the analysis of live cells on a single-cell basis and offers a rapid, efficient and high-throughput (up to 234,000 individual cells) system for identifying and recovering objective ASCs. We applied the system to detect and retrieve ASCs for hepatitis B virus and influenza viruses from human peripheral blood lymphocytes and produced human mAbs with virus-neutralizing activities within a week. Furthermore, we show that the system is useful for detecting ASCs for multiple antigens as well as for selection of ASCs secreting high-affinity antibodies on a chip. Our method can open the way for the generation of therapeutic antibodies for individual patients. IS - 9 JF - Nature Medicine SN - 1078-8956 EP - 1092 TI - A rapid and efficient single-cell manipulation method for screening antigen-specific antibody|[ndash]|secreting cells from human peripheral blood PB - Nature Publishing Group VL - 15 SP - 1088 KW - fourstar KW - immunology KW - method KW - microfluidics KW - multiplexing KW - screening AU - Jin, Aishun AU - Ozawa, Tatsuhiko AU - Tajiri, Kazuto AU - Obata, Tsutomu AU - Kondo, Sachiko AU - Kinoshita, Koshi AU - Kadowaki, Shinichi AU - Takahashi, Kazuo AU - Sugiyama, Toshiro AU - Kishi, Hiroyuki AU - Muraguchi, Atsushi PY - 2009/08/16/ UR - http://dx.doi.org/10.1038/nm.1966 ER -