Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast Export

@article{citeulike:5518679, abstract = {We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid and then transplanted it into Mycoplasma capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered by using yeast genetic systems and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium. 10.1126/science.1173759}, author = {Lartigue, Carole and Vashee, Sanjay and Algire, Mikkel A. and Chuang, Ray-Yuan and Benders, Gwynedd A. and Ma, Li and Noskov, Vladimir N. and Denisova, Evgeniya A. and Gibson, Daniel G. and Assad-Garcia, Nacyra and Alperovich, Nina and Thomas, David W. and Merryman, Chuck and Hutchison, Clyde A. and Smith, Hamilton O. and Venter, J. Craig and Glass, John I.}, citeulike-article-id = {5518679}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1173759}, citeulike-linkout-1 = {http://www.sciencemag.org/content/325/5948/1693.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/325/5948/1693.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19696314}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19696314}, day = {25}, doi = {10.1126/science.1173759}, journal = {Science}, keywords = {engineering, fivestar, geneticmod, journalclub, method}, month = {September}, number = {5948}, pages = {1693--1696}, posted-at = {2009-08-21 16:41:53}, priority = {2}, title = {Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast}, url = {http://dx.doi.org/10.1126/science.1173759}, volume = {325}, year = {2009} }

TY - JOUR ID - citeulike:5518679 L3 - citeulike-article-id:5518679 N2 - We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid and then transplanted it into Mycoplasma capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered by using yeast genetic systems and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium. 10.1126/science.1173759 IS - 5948 JF - Science EP - 1696 TI - Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast VL - 325 SP - 1693 KW - engineering KW - fivestar KW - geneticmod KW - journalclub KW - method AU - Lartigue, Carole AU - Vashee, Sanjay AU - Algire, Mikkel AU - Chuang, Ray-Yuan AU - Benders, Gwynedd AU - Ma, Li AU - Noskov, Vladimir AU - Denisova, Evgeniya AU - Gibson, Daniel AU - Assad-Garcia, Nacyra AU - Alperovich, Nina AU - Thomas, David AU - Merryman, Chuck AU - Hutchison, Clyde AU - Smith, Hamilton AU - Venter, Craig AU - Glass, John PY - 2009/09/25/ UR - http://dx.doi.org/10.1126/science.1173759 ER -