Imaging oxygen consumption in forepaw somatosensory stimulation in rats under isoflurane anesthesia. Export

@article{citeulike:838459, abstract = {The cerebral metabolic rate of oxygen (CMRO2) was dynamically evaluated on a pixel-by-pixel basis in isoflurane-anesthetized and spontaneously breathing rats following graded electrical somatosensory forepaw stimulations (4, 6, and 8 mA). In contrast to alpha-chloralose, which is the most widely used anesthetic in forepaw-stimulation fMRI studies of rats under mechanical ventilation, isoflurane (1.1-1.2\%) provided a stable anesthesia level over a prolonged period, without the need to adjust the ventilation volume/rate or sample blood gases. Combined cerebral blood flow signals (CBF) and blood oxygenation level-dependent (BOLD) fMRI signals were simultaneously measured with the use of a multislice continuous arterial spin labeling (CASL) technique (two-coil setup). CMRO2 was calculated using the biophysical BOLD model of Ogawa et al. (Proc Natl Acad Sci USA 1992;89:5951-5955). The stimulus-evoked BOLD percent changes at 4, 6, and 8 A were, respectively, 0.5\% +/- 0.2\%, 1.4\% +/- 0.3\%, and 2.0\% +/- 0.3\% (mean +/- SD, N = 6). The CBF percent changes were 23\% +/- 6\%, 58\% +/- 9\%, and 87\% +/- 14\%. The CMRO2 percent changes were 14\% +/- 4\%, 24\% +/- 6\%, and 43\% +/- 11\%. BOLD, CBF, and CMRO2 activations were localized to the forepaw somatosensory cortices without evidence of plateau for oxygen consumption, indicative of partial coupling of CBF and CMRO2. This study describes a useful forepaw-stimulation model for fMRI, and demonstrate that CMRO2 changes can be dynamically imaged on a pixel-by-pixel basis in a single setting with high spatiotemporal resolution.}, address = {Center for Comparative NeuroImaging, Department of Psychiatry, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA. timothy.duong@umassmed.edu}, author = {Liu, Z. M. and Schmidt, K. F. and Sicard, K. M. and Duong, T. Q.}, citeulike-article-id = {838459}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/mrm.20148}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15282809}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15282809}, comment = {model on anaesthetic animal (isoflurane) for BOLD, CBF and CMRO2 variations related to forepaw stimulations.}, doi = {10.1002/mrm.20148}, issn = {0740-3194}, journal = {Magn Reson Med}, keywords = {anaesthesia, animal, bold, fmri, perfusion}, month = {August}, number = {2}, pages = {277--285}, posted-at = {2006-09-10 18:32:11}, priority = {2}, title = {Imaging oxygen consumption in forepaw somatosensory stimulation in rats under isoflurane anesthesia.}, url = {http://dx.doi.org/10.1002/mrm.20148}, volume = {52}, year = {2004} }

TY - JOUR ID - citeulike:838459 L3 - citeulike-article-id:838459 N2 - The cerebral metabolic rate of oxygen (CMRO2) was dynamically evaluated on a pixel-by-pixel basis in isoflurane-anesthetized and spontaneously breathing rats following graded electrical somatosensory forepaw stimulations (4, 6, and 8 mA). In contrast to alpha-chloralose, which is the most widely used anesthetic in forepaw-stimulation fMRI studies of rats under mechanical ventilation, isoflurane (1.1-1.2%) provided a stable anesthesia level over a prolonged period, without the need to adjust the ventilation volume/rate or sample blood gases. Combined cerebral blood flow signals (CBF) and blood oxygenation level-dependent (BOLD) fMRI signals were simultaneously measured with the use of a multislice continuous arterial spin labeling (CASL) technique (two-coil setup). CMRO2 was calculated using the biophysical BOLD model of Ogawa et al. (Proc Natl Acad Sci USA 1992;89:5951-5955). The stimulus-evoked BOLD percent changes at 4, 6, and 8 A were, respectively, 0.5% +/- 0.2%, 1.4% +/- 0.3%, and 2.0% +/- 0.3% (mean +/- SD, N = 6). The CBF percent changes were 23% +/- 6%, 58% +/- 9%, and 87% +/- 14%. The CMRO2 percent changes were 14% +/- 4%, 24% +/- 6%, and 43% +/- 11%. BOLD, CBF, and CMRO2 activations were localized to the forepaw somatosensory cortices without evidence of plateau for oxygen consumption, indicative of partial coupling of CBF and CMRO2. This study describes a useful forepaw-stimulation model for fMRI, and demonstrate that CMRO2 changes can be dynamically imaged on a pixel-by-pixel basis in a single setting with high spatiotemporal resolution. IS - 2 JF - Magn Reson Med SN - 0740-3194 AD - Center for Comparative NeuroImaging, Department of Psychiatry, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA. timothy.duong@umassmed.edu EP - 285 TI - Imaging oxygen consumption in forepaw somatosensory stimulation in rats under isoflurane anesthesia. VL - 52 SP - 277 KW - anaesthesia KW - animal KW - bold KW - fmri KW - perfusion N1 - model on anaesthetic animal (isoflurane) for BOLD, CBF and CMRO2 variations related to forepaw stimulations. AU - Liu, ZM AU - Schmidt, KF AU - Sicard, KM AU - Duong, TQ PY - 2004/08// UR - http://dx.doi.org/10.1002/mrm.20148 ER -