Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins. Export

@article{citeulike:2845991, abstract = {The immunosuppressant rapamycin inhibits Tor1p and Tor2p (target of rapamycin proteins), ultimately resulting in cellular responses characteristic of nutrient deprivation through a mechanism involving translational arrest. We measured the immediate transcriptional response of yeast grown in rich media and treated with rapamycin to investigate the direct effects of Tor proteins on nutrient-sensitive signaling pathways. The results suggest that Tor proteins directly modulate the glucose activation and nitrogen discrimination pathways and the pathways that respond to the diauxic shift (including glycolysis and the citric acid cycle). Tor proteins do not directly modulate the general amino acid control, nitrogen starvation, or sporulation (in diploid cells) pathways. Poor nitrogen quality activates the nitrogen discrimination pathway, which is controlled by the complex of the transcriptional repressor Ure2p and activator Gln3p. Inhibiting Tor proteins with rapamycin increases the electrophoretic mobility of Ure2p. The work presented here illustrates the coordinated use of genome-based and biochemical approaches to delineate a cellular pathway modulated by the protein target of a small molecule.}, address = {Howard Hughes Medical Institute, Harvard Center for Genomics Research, Department of Chemistry, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.}, author = {Hardwick, J. S. and Kuruvilla, F. G. and Tong, J. K. and Shamji, A. F. and Schreiber, S. L.}, citeulike-article-id = {2845991}, citeulike-linkout-0 = {http://www.pnas.org/content/96/26/14866.full.abstract}, citeulike-linkout-1 = {http://www.pnas.org/content/96/26/14866.full.full.pdf}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/10611304}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=10611304}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {cell\_biology, yeast}, month = {December}, number = {26}, pages = {14866--14870}, posted-at = {2009-06-03 00:59:03}, priority = {2}, title = {Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins.}, url = {http://www.pnas.org/content/96/26/14866.full.abstract}, volume = {96}, year = {1999} }

TY - JOUR ID - citeulike:2845991 L3 - citeulike-article-id:2845991 N2 - The immunosuppressant rapamycin inhibits Tor1p and Tor2p (target of rapamycin proteins), ultimately resulting in cellular responses characteristic of nutrient deprivation through a mechanism involving translational arrest. We measured the immediate transcriptional response of yeast grown in rich media and treated with rapamycin to investigate the direct effects of Tor proteins on nutrient-sensitive signaling pathways. The results suggest that Tor proteins directly modulate the glucose activation and nitrogen discrimination pathways and the pathways that respond to the diauxic shift (including glycolysis and the citric acid cycle). Tor proteins do not directly modulate the general amino acid control, nitrogen starvation, or sporulation (in diploid cells) pathways. Poor nitrogen quality activates the nitrogen discrimination pathway, which is controlled by the complex of the transcriptional repressor Ure2p and activator Gln3p. Inhibiting Tor proteins with rapamycin increases the electrophoretic mobility of Ure2p. The work presented here illustrates the coordinated use of genome-based and biochemical approaches to delineate a cellular pathway modulated by the protein target of a small molecule. IS - 26 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Howard Hughes Medical Institute, Harvard Center for Genomics Research, Department of Chemistry, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA. EP - 14870 TI - Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins. VL - 96 SP - 14866 KW - cell_biology KW - yeast AU - Hardwick, JS AU - Kuruvilla, FG AU - Tong, JK AU - Shamji, AF AU - Schreiber, SL PY - 1999/12/21/ UR - http://www.pnas.org/content/96/26/14866.full.abstract ER -