A high-resolution linkage map of the vicinity of the rice submergence tolerance locus Sub1. Export

@article{citeulike:4925006, abstract = {Resistance to submergence stress is an important breeding objective in areas where rice cultivars are subjected to complete inundation for a week or more. The present study was conducted to develop a high-resolution map of the region surrounding the submergence tolerance gene Sub1 in rice, which derives from the Indian cultivar FR13A. Submergence screening of 8-day-old plants of F3 families kept for 14 days submerged in 60 cm of water allowed an accurate classification of Sub1 phenotypes. Bulked segregant analysis was used to identify AFLP markers linked to Sub1. A population of 2950 F2 plants segregating for Sub1 was screened with two RFLP markers flanking the Sub1 locus, 2.4 and 4.9 cM away. Submergence tolerance was measured in the recombinant plants, and AFLP markers closely linked to Sub1 were mapped. Two AFLP markers cosegregated with Sub1 in this large population, and other markers were localized within 0.2 cM of Sub1. The high-resolution map should serve as the basis for map-based cloning of this important locus, as it will permit the identification of BAC clones spanning the region.}, author = {Xu, K. and Xu, X. and Ronald, P. C. and Mackill, D. J.}, citeulike-article-id = {4925006}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/10852491}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=10852491}, issn = {0026-8925}, journal = {Molecular \& general genetics : MGG}, keywords = {network\_engineering}, month = {May}, number = {4}, pages = {681--689}, posted-at = {2009-06-22 18:03:43}, priority = {3}, title = {A high-resolution linkage map of the vicinity of the rice submergence tolerance locus Sub1.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10852491}, volume = {263}, year = {2000} }

TY - JOUR ID - citeulike:4925006 L3 - citeulike-article-id:4925006 N2 - Resistance to submergence stress is an important breeding objective in areas where rice cultivars are subjected to complete inundation for a week or more. The present study was conducted to develop a high-resolution map of the region surrounding the submergence tolerance gene Sub1 in rice, which derives from the Indian cultivar FR13A. Submergence screening of 8-day-old plants of F3 families kept for 14 days submerged in 60 cm of water allowed an accurate classification of Sub1 phenotypes. Bulked segregant analysis was used to identify AFLP markers linked to Sub1. A population of 2950 F2 plants segregating for Sub1 was screened with two RFLP markers flanking the Sub1 locus, 2.4 and 4.9 cM away. Submergence tolerance was measured in the recombinant plants, and AFLP markers closely linked to Sub1 were mapped. Two AFLP markers cosegregated with Sub1 in this large population, and other markers were localized within 0.2 cM of Sub1. The high-resolution map should serve as the basis for map-based cloning of this important locus, as it will permit the identification of BAC clones spanning the region. IS - 4 JF - Molecular & general genetics : MGG SN - 0026-8925 EP - 689 TI - A high-resolution linkage map of the vicinity of the rice submergence tolerance locus Sub1. VL - 263 SP - 681 KW - network_engineering AU - Xu, K AU - Xu, X AU - Ronald, PC AU - Mackill, DJ PY - 2000/05// UR - http://view.ncbi.nlm.nih.gov/pubmed/10852491 ER -