Dynamic Proteomics: a database for dynamics and localizations of endogenous fluorescently-tagged proteins in living human cells Export

@article{citeulike:5986000, abstract = {Recent advances allow tracking the levels and locations of a thousand proteins in individual living human cells over time using a library of annotated reporter cell clones (LARC). This library was created by Cohen et al. to study the proteome dynamics of a human lung carcinoma cell-line treated with an anti-cancer drug. Here, we report the Dynamic Proteomics database for the proteins studied by Cohen et al. Each cell-line clone in LARC has a protein tagged with yellow fluorescent protein, expressed from its endogenous chromosomal location, under its natural regulation. The Dynamic Proteomics interface facilitates searches for genes of interest, downloads of protein fluorescent movies and alignments of dynamics following drug addition. Each protein in the database is displayed with its annotation, cDNA sequence, fluorescent images and movies obtained by the time-lapse microscopy. The protein dynamics in the database represents a quantitative trace of the protein fluorescence levels in nucleus and cytoplasm produced by image analysis of movies over time. Furthermore, a sequence analysis provides a search and comparison of up to 50 input DNA sequences with all cDNAs in the library. The raw movies may be useful as a benchmark for developing image analysis tools for individual-cell dynamic-proteomics. The database is available at http://www.dynamicproteomics.net/. 10.1093/nar/gkp808}, author = {Frenkel-Morgenstern, Milana and Cohen, Ariel A. and Geva-Zatorsky, Naama and Eden, Eran and Prilusky, Jaime and Issaeva, Irina and Sigal, Alex and Cohen-Saidon, Cellina and Liron, Yuvalal and Cohen, Lydia and Danon, Tamar and Perzov, Natalie and Alon, Uri}, citeulike-article-id = {5986000}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkp808}, citeulike-linkout-1 = {http://nar.oxfordjournals.org/content/gkp808v1/.abstract}, citeulike-linkout-2 = {http://nar.oxfordjournals.org/content/gkp808v1/.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19820112}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19820112}, day = {9}, doi = {10.1093/nar/gkp808}, journal = {Nucl. Acids Res.}, keywords = {cell\_biology, fluorescence, human}, month = {October}, pages = {gkp808+}, posted-at = {2009-10-22 17:30:39}, priority = {2}, title = {Dynamic Proteomics: a database for dynamics and localizations of endogenous fluorescently-tagged proteins in living human cells}, url = {http://dx.doi.org/10.1093/nar/gkp808}, year = {2009} }

TY - JOUR ID - citeulike:5986000 L3 - citeulike-article-id:5986000 N2 - Recent advances allow tracking the levels and locations of a thousand proteins in individual living human cells over time using a library of annotated reporter cell clones (LARC). This library was created by Cohen et al. to study the proteome dynamics of a human lung carcinoma cell-line treated with an anti-cancer drug. Here, we report the Dynamic Proteomics database for the proteins studied by Cohen et al. Each cell-line clone in LARC has a protein tagged with yellow fluorescent protein, expressed from its endogenous chromosomal location, under its natural regulation. The Dynamic Proteomics interface facilitates searches for genes of interest, downloads of protein fluorescent movies and alignments of dynamics following drug addition. Each protein in the database is displayed with its annotation, cDNA sequence, fluorescent images and movies obtained by the time-lapse microscopy. The protein dynamics in the database represents a quantitative trace of the protein fluorescence levels in nucleus and cytoplasm produced by image analysis of movies over time. Furthermore, a sequence analysis provides a search and comparison of up to 50 input DNA sequences with all cDNAs in the library. The raw movies may be useful as a benchmark for developing image analysis tools for individual-cell dynamic-proteomics. The database is available at http://www.dynamicproteomics.net/. 10.1093/nar/gkp808 JF - Nucl. Acids Res. TI - Dynamic Proteomics: a database for dynamics and localizations of endogenous fluorescently-tagged proteins in living human cells SP - gkp808 KW - cell_biology KW - fluorescence KW - human AU - Frenkel-Morgenstern, Milana AU - Cohen, Ariel AU - Geva-Zatorsky, Naama AU - Eden, Eran AU - Prilusky, Jaime AU - Issaeva, Irina AU - Sigal, Alex AU - Cohen-Saidon, Cellina AU - Liron, Yuvalal AU - Cohen, Lydia AU - Danon, Tamar AU - Perzov, Natalie AU - Alon, Uri PY - 2009/10/09/ UR - http://dx.doi.org/10.1093/nar/gkp808 ER -