A survey of genetic human cortical gene expression. Export

@article{citeulike:1890028, abstract = {It is widely assumed that genetic differences in gene expression underpin much of the difference among individuals and many of the quantitative traits of interest to geneticists. Despite this, there has been little work on genetic variability in human gene expression and almost none in the human brain, because tools for assessing this genetic variability have not been available. Now, with whole-genome SNP genotyping arrays and whole-transcriptome expression arrays, such experiments have become feasible. We have carried out whole-genome genotyping and expression analysis on a series of 193 neuropathologically normal human brain samples using the Affymetrix GeneChip Human Mapping 500K Array Set and Illumina HumanRefseq-8 Expression BeadChip platforms. Here we present data showing that 58\% of the transcriptome is cortically expressed in at least 5\% of our samples and that of these cortically expressed transcripts, 21\% have expression profiles that correlate with their genotype. These genetic-expression effects should be useful in determining the underlying biology of associations with common diseases of the human brain and in guiding the analysis of the genomic regions involved in the control of normal gene expression.}, address = {[1] Laboratory of Neurogenetics, National Institute on Aging, Porter Neuroscience Building, National Institutes of Health Main Campus, Bethesda, Maryland 20892, USA. [2] Department of Psychiatry and Behavioral Sciences, Division of Neuroscience, Miller School of Medicine, University of Miami, Batchelors Children's Research Building, Room 609, 1580 10th Avenue, Miami, Florida 33136, USA. [3] These authors contributed equally to this work.}, author = {Myers, Amanda J. and Gibbs, J. Raphael and Webster, Jennifer A. and Rohrer, Kristen and Zhao, Alice and Marlowe, Lauren and Kaleem, Mona and Leung, Doris and Bryden, Leslie and Nath, Priti and Zismann, Victoria L. and Joshipura, Keta and Huentelman, Matthew J. and Hu-Lince, Diane and Coon, Keith D. and Craig, David W. and Pearson, John V. and Holmans, Peter and Heward, Christopher B. and Reiman, Eric M. and Stephan, Dietrich and Hardy, John}, citeulike-article-id = {1890028}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ng.2007.16}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/ng.2007.16}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17982457}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17982457}, day = {4}, doi = {10.1038/ng.2007.16}, issn = {1546-1718}, journal = {Nature genetics}, keywords = {mouse\_human}, month = {December}, number = {12}, pages = {1494--1499}, posted-at = {2008-09-25 19:36:19}, priority = {2}, title = {A survey of genetic human cortical gene expression.}, url = {http://dx.doi.org/10.1038/ng.2007.16}, volume = {39}, year = {2007} }

TY - JOUR ID - citeulike:1890028 L3 - citeulike-article-id:1890028 N2 - It is widely assumed that genetic differences in gene expression underpin much of the difference among individuals and many of the quantitative traits of interest to geneticists. Despite this, there has been little work on genetic variability in human gene expression and almost none in the human brain, because tools for assessing this genetic variability have not been available. Now, with whole-genome SNP genotyping arrays and whole-transcriptome expression arrays, such experiments have become feasible. We have carried out whole-genome genotyping and expression analysis on a series of 193 neuropathologically normal human brain samples using the Affymetrix GeneChip Human Mapping 500K Array Set and Illumina HumanRefseq-8 Expression BeadChip platforms. Here we present data showing that 58% of the transcriptome is cortically expressed in at least 5% of our samples and that of these cortically expressed transcripts, 21% have expression profiles that correlate with their genotype. These genetic-expression effects should be useful in determining the underlying biology of associations with common diseases of the human brain and in guiding the analysis of the genomic regions involved in the control of normal gene expression. IS - 12 JF - Nature genetics SN - 1546-1718 AD - [1] Laboratory of Neurogenetics, National Institute on Aging, Porter Neuroscience Building, National Institutes of Health Main Campus, Bethesda, Maryland 20892, USA. [2] Department of Psychiatry and Behavioral Sciences, Division of Neuroscience, Miller School of Medicine, University of Miami, Batchelors Children's Research Building, Room 609, 1580 10th Avenue, Miami, Florida 33136, USA. [3] These authors contributed equally to this work. EP - 1499 TI - A survey of genetic human cortical gene expression. VL - 39 SP - 1494 KW - mouse_human AU - Myers, Amanda AU - Gibbs, Raphael AU - Webster, Jennifer AU - Rohrer, Kristen AU - Zhao, Alice AU - Marlowe, Lauren AU - Kaleem, Mona AU - Leung, Doris AU - Bryden, Leslie AU - Nath, Priti AU - Zismann, Victoria AU - Joshipura, Keta AU - Huentelman, Matthew AU - Hu-Lince, Diane AU - Coon, Keith AU - Craig, David AU - Pearson, John AU - Holmans, Peter AU - Heward, Christopher AU - Reiman, Eric AU - Stephan, Dietrich AU - Hardy, John PY - 2007/12/04/ UR - http://dx.doi.org/10.1038/ng.2007.16 ER -