A translational profiling approach for the molecular characterization of CNS cell types. Export

@article{citeulike:3574510, abstract = {The cellular heterogeneity of the brain confounds efforts to elucidate the biological properties of distinct neuronal populations. Using bacterial artificial chromosome (BAC) transgenic mice that express EGFP-tagged ribosomal protein L10a in defined cell populations, we have developed a methodology for affinity purification of polysomal mRNAs from genetically defined cell populations in the brain. The utility of this approach is illustrated by the comparative analysis of four types of neurons, revealing hundreds of genes that distinguish these four cell populations. We find that even two morphologically indistinguishable, intermixed subclasses of medium spiny neurons display vastly different translational profiles and present examples of the physiological significance of such differences. This genetically targeted translating ribosome affinity purification (TRAP) methodology is a generalizable method useful for the identification of molecular changes in any genetically defined cell type in response to genetic alterations, disease, or pharmacological perturbations.}, author = {Heiman, Myriam and Schaefer, Anne and Gong, Shiaoching and Peterson, Jayms D. and Day, Michelle and Ramsey, Keri E. and Su\'{a}rez-Fari\~{n}as, Mayte and Schwarz, Cordelia and Stephan, Dietrich A. and Surmeier, D. James and Greengard, Paul and Heintz, Nathaniel}, citeulike-article-id = {3574510}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2008.10.028}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0092867408013652}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19013281}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19013281}, day = {14}, doi = {10.1016/j.cell.2008.10.028}, issn = {1097-4172}, journal = {Cell}, keywords = {cell\_populations}, month = {November}, number = {4}, pages = {738--748}, posted-at = {2009-09-09 00:29:26}, priority = {2}, title = {A translational profiling approach for the molecular characterization of CNS cell types.}, url = {http://dx.doi.org/10.1016/j.cell.2008.10.028}, volume = {135}, year = {2008} }

TY - JOUR ID - citeulike:3574510 L3 - citeulike-article-id:3574510 N2 - The cellular heterogeneity of the brain confounds efforts to elucidate the biological properties of distinct neuronal populations. Using bacterial artificial chromosome (BAC) transgenic mice that express EGFP-tagged ribosomal protein L10a in defined cell populations, we have developed a methodology for affinity purification of polysomal mRNAs from genetically defined cell populations in the brain. The utility of this approach is illustrated by the comparative analysis of four types of neurons, revealing hundreds of genes that distinguish these four cell populations. We find that even two morphologically indistinguishable, intermixed subclasses of medium spiny neurons display vastly different translational profiles and present examples of the physiological significance of such differences. This genetically targeted translating ribosome affinity purification (TRAP) methodology is a generalizable method useful for the identification of molecular changes in any genetically defined cell type in response to genetic alterations, disease, or pharmacological perturbations. IS - 4 JF - Cell SN - 1097-4172 EP - 748 TI - A translational profiling approach for the molecular characterization of CNS cell types. VL - 135 SP - 738 KW - cell_populations AU - Heiman, Myriam AU - Schaefer, Anne AU - Gong, Shiaoching AU - Peterson, Jayms AU - Day, Michelle AU - Ramsey, Keri AU - Suárez-Fariñas, Mayte AU - Schwarz, Cordelia AU - Stephan, Dietrich AU - Surmeier, James AU - Greengard, Paul AU - Heintz, Nathaniel PY - 2008/11/14/ UR - http://dx.doi.org/10.1016/j.cell.2008.10.028 ER -