Circulating microRNAs as stable blood-based markers for cancer detection Export

@article{citeulike:3090225, abstract = {10.1073/pnas.0804549105 Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small (≈22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer.}, address = {Divisions of Human Biology, Clinical Research, and Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.}, author = {Mitchell, Patrick S. and Parkin, Rachael K. and Kroh, Evan M. and Fritz, Brian R. and Wyman, Stacia K. and Pogosova-Agadjanyan, Era L. and Peterson, Amelia and Noteboom, Jennifer and O'Briant, Kathy C. and Allen, April and Lin, Daniel W. and Urban, Nicole and Drescher, Charles W. and Knudsen, Beatrice S. and Stirewalt, Derek L. and Gentleman, Robert and Vessella, Robert L. and Nelson, Peter S. and Martin, Daniel B. and Tewari, Muneesh}, citeulike-article-id = {3090225}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0804549105}, citeulike-linkout-1 = {http://www.pnas.org/content/105/30/10513.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/30/10513.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/18663219}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=18663219}, day = {29}, doi = {10.1073/pnas.0804549105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences}, keywords = {marker, mirna, serum}, month = {July}, number = {30}, pages = {10513--10518}, posted-at = {2008-09-16 09:21:34}, priority = {0}, title = {Circulating microRNAs as stable blood-based markers for cancer detection}, url = {http://dx.doi.org/10.1073/pnas.0804549105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:3090225 L3 - citeulike-article-id:3090225 N2 - 10.1073/pnas.0804549105 Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small (≈22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer. IS - 30 JF - Proceedings of the National Academy of Sciences SN - 1091-6490 AD - Divisions of Human Biology, Clinical Research, and Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. EP - 10518 TI - Circulating microRNAs as stable blood-based markers for cancer detection VL - 105 SP - 10513 KW - marker KW - mirna KW - serum AU - Mitchell, Patrick AU - Parkin, Rachael AU - Kroh, Evan AU - Fritz, Brian AU - Wyman, Stacia AU - Pogosova-Agadjanyan, Era AU - Peterson, Amelia AU - Noteboom, Jennifer AU - O'Briant, Kathy AU - Allen, April AU - Lin, Daniel AU - Urban, Nicole AU - Drescher, Charles AU - Knudsen, Beatrice AU - Stirewalt, Derek AU - Gentleman, Robert AU - Vessella, Robert AU - Nelson, Peter AU - Martin, Daniel AU - Tewari, Muneesh PY - 2008/07/29/ UR - http://dx.doi.org/10.1073/pnas.0804549105 ER -