Multiple putative oncogenes at the chromosome 20q amplicon contribute to colorectal adenoma to carcinoma progression. Export

@article{citeulike:3708846, abstract = {OBJECTIVE: This study aimed to identify the oncogenes at 20q involved in colorectal adenoma to carcinoma progression by measuring the effect of 20q gain on mRNA expression of genes in this amplicon. METHODS: Segmentation of DNA copy number changes on 20q was performed by array CGH in 34 non-progressed colorectal adenomas, 41 progressed adenomas (i.e. adenomas that present a focus of cancer) and 33 adenocarcinomas. Moreover, a robust analysis of altered expression of genes in these segments was performed by microarray analysis in 37 adenomas and 31 adenocarcinomas. Protein expression was evaluated by immunohistochemistry on tissue microarrays. RESULTS: Genes C20orf24, AURKA, RNPC1, TH1L, ADRM1, C20orf20 and TCFL5, mapping at 20q were significantly overexpressed in carcinomas compared to adenomas as consequence of copy number gain of 20q. CONCLUSION: This approach revealed C20orf24, AURKA, RNPC1, TH1L, ADRM1, C20orf20 and TCFL5 genes to be important in chromosomal instability-related adenoma to carcinoma progression. These genes therefore may serve as highly specific biomarkers for colorectal cancer with potential clinical applications.}, author = {Carvalho, Beatriz and Postma, Cindy and Mongera, Sandra and Hopmans, Erik and Diskin, Sharon and van de Wiel, Mark A. A. and van Criekinge, Wim and Thas, Olivier and Matth\"{a}i, Anja and Cuesta, Miguel A. A. and Jochim and Mikael and Schr\"{o}ck, Evelin and Ylstra, Bauke and Meijer, Gerrit A. A.}, citeulike-article-id = {3708846}, citeulike-linkout-0 = {http://dx.doi.org/10.1136/gut.2007.143065}, citeulike-linkout-1 = {http://gut.bmj.com/cgi/content/abstract/58/1/79}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18829976}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18829976}, day = {12}, doi = {10.1136/gut.2007.143065}, issn = {1468-3288}, journal = {Gut}, keywords = {20q, array-cgh, colon\_cancer, crc\_bib, gene\_expression, integration}, month = {January}, number = {1}, pages = {79--89}, posted-at = {2008-11-26 17:48:39}, priority = {0}, title = {Multiple putative oncogenes at the chromosome 20q amplicon contribute to colorectal adenoma to carcinoma progression.}, url = {http://dx.doi.org/10.1136/gut.2007.143065}, volume = {58}, year = {2009} }

TY - JOUR ID - citeulike:3708846 L3 - citeulike-article-id:3708846 N2 - OBJECTIVE: This study aimed to identify the oncogenes at 20q involved in colorectal adenoma to carcinoma progression by measuring the effect of 20q gain on mRNA expression of genes in this amplicon. METHODS: Segmentation of DNA copy number changes on 20q was performed by array CGH in 34 non-progressed colorectal adenomas, 41 progressed adenomas (i.e. adenomas that present a focus of cancer) and 33 adenocarcinomas. Moreover, a robust analysis of altered expression of genes in these segments was performed by microarray analysis in 37 adenomas and 31 adenocarcinomas. Protein expression was evaluated by immunohistochemistry on tissue microarrays. RESULTS: Genes C20orf24, AURKA, RNPC1, TH1L, ADRM1, C20orf20 and TCFL5, mapping at 20q were significantly overexpressed in carcinomas compared to adenomas as consequence of copy number gain of 20q. CONCLUSION: This approach revealed C20orf24, AURKA, RNPC1, TH1L, ADRM1, C20orf20 and TCFL5 genes to be important in chromosomal instability-related adenoma to carcinoma progression. These genes therefore may serve as highly specific biomarkers for colorectal cancer with potential clinical applications. IS - 1 JF - Gut SN - 1468-3288 EP - 89 TI - Multiple putative oncogenes at the chromosome 20q amplicon contribute to colorectal adenoma to carcinoma progression. VL - 58 SP - 79 KW - 20q KW - array-cgh KW - colon_cancer KW - crc_bib KW - gene_expression KW - integration AU - Carvalho, Beatriz AU - Postma, Cindy AU - Mongera, Sandra AU - Hopmans, Erik AU - Diskin, Sharon AU - van de Wiel, Mark AU - van Criekinge, Wim AU - Thas, Olivier AU - Matthäi, Anja AU - Cuesta, Miguel AU - Jochim AU - Mikael AU - Schröck, Evelin AU - Ylstra, Bauke AU - Meijer, Gerrit PY - 2009/01/12/ UR - http://dx.doi.org/10.1136/gut.2007.143065 ER -