The Antisense Transcriptomes of Human Cells

@article{citeulike:3749873, abstract = {Transcription in mammalian cells can be assessed at a genome-wide level, but it has been difficult to reliably determine whether individual transcripts are derived from the plus- or minus-strands of chromosomes. This distinction can be critical for understanding the relationship between known transcripts (sense) and the complementary antisense transcripts that may regulate them. Here, we describe a technique that can be used to (i) identify the DNA strand of origin for any particular RNA transcript and (ii) quantify the number of sense and antisense transcripts from expressed genes at a global level. We examined five different human cell types and in each case found evidence for antisense transcripts in 2900 to 6400 human genes. The distribution of antisense transcripts was distinct from that of sense transcripts, was nonrandom across the genome, and differed among cell types. Antisense transcripts thus appear to be a pervasive feature of human cells, suggesting that they are a fundamental component of gene regulation. 10.1126/science.1163853}, author = {He, Yiping and Vogelstein, Bert and Velculescu, Victor E. and Papadopoulos, Nickolas and Kinzler, Kenneth W.}, citeulike-article-id = {3749873}, doi = {10.1126/science.1163853}, journal = {Science}, keywords = {antisense}, month = {December}, pages = {1163853+}, posted-at = {2009-01-10 11:06:15}, priority = {0}, title = {The Antisense Transcriptomes of Human Cells}, url = {http://dx.doi.org/10.1126/science.1163853}, year = {2008} }

TY - JOUR ID - citeulike:3749873 L3 - citeulike-article-id:3749873 N2 - Transcription in mammalian cells can be assessed at a genome-wide level, but it has been difficult to reliably determine whether individual transcripts are derived from the plus- or minus-strands of chromosomes. This distinction can be critical for understanding the relationship between known transcripts (sense) and the complementary antisense transcripts that may regulate them. Here, we describe a technique that can be used to (i) identify the DNA strand of origin for any particular RNA transcript and (ii) quantify the number of sense and antisense transcripts from expressed genes at a global level. We examined five different human cell types and in each case found evidence for antisense transcripts in 2900 to 6400 human genes. The distribution of antisense transcripts was distinct from that of sense transcripts, was nonrandom across the genome, and differed among cell types. Antisense transcripts thus appear to be a pervasive feature of human cells, suggesting that they are a fundamental component of gene regulation. 10.1126/science.1163853 JF - Science TI - The Antisense Transcriptomes of Human Cells SP - 1163853 KW - antisense AU - He, Yiping AU - Vogelstein, Bert AU - Velculescu, Victor AU - Papadopoulos, Nickolas AU - Kinzler, Kenneth PY - 2008/12/04/ UR - http://dx.doi.org/10.1126/science.1163853 ER -