Expression patterns of vascular-specific promoters RolC and Sh in transgenic potatoes and their use in engineering PLRV-resistant plants. Export

@article{citeulike:591033, abstract = {The expression patterns of GUS fusion constructs driven by the Agrobacterium rhizogenes RolC and the maize Sh (Shrunken; sucrose synthase-1) promoters were examined in transgenic potatoes (cv. Atlantic). RolC drove high-level gene expression in phloem tissue, bundle sheath cells and vascular parenchyma, but not in xylem or non-vascular tissues. Sh expression was exclusively confined to phloem tissue. Potato leafroll luteovirus (PLRV) replicates only in phloem tissues, and we show that when RolC is used to drive expression of the PLRV coat protein gene, virus-resistant lines can be obtained. In contrast, no significant resistance was observed when the Sh promoter was used.}, address = {CSIRO Division of Plant Industry, Canberra, Australia.}, author = {Graham, M. W. and Craig, S. and Waterhouse, P. M.}, citeulike-article-id = {591033}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9132064}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9132064}, issn = {0167-4412}, journal = {Plant Mol Biol}, keywords = {phloem, promoter}, month = {March}, number = {4}, pages = {729--735}, posted-at = {2006-04-19 09:33:58}, priority = {2}, title = {Expression patterns of vascular-specific promoters RolC and Sh in transgenic potatoes and their use in engineering PLRV-resistant plants.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9132064}, volume = {33}, year = {1997} }

TY - JOUR ID - citeulike:591033 L3 - citeulike-article-id:591033 N2 - The expression patterns of GUS fusion constructs driven by the Agrobacterium rhizogenes RolC and the maize Sh (Shrunken; sucrose synthase-1) promoters were examined in transgenic potatoes (cv. Atlantic). RolC drove high-level gene expression in phloem tissue, bundle sheath cells and vascular parenchyma, but not in xylem or non-vascular tissues. Sh expression was exclusively confined to phloem tissue. Potato leafroll luteovirus (PLRV) replicates only in phloem tissues, and we show that when RolC is used to drive expression of the PLRV coat protein gene, virus-resistant lines can be obtained. In contrast, no significant resistance was observed when the Sh promoter was used. IS - 4 JF - Plant Mol Biol SN - 0167-4412 AD - CSIRO Division of Plant Industry, Canberra, Australia. EP - 735 TI - Expression patterns of vascular-specific promoters RolC and Sh in transgenic potatoes and their use in engineering PLRV-resistant plants. VL - 33 SP - 729 KW - phloem KW - promoter AU - Graham, MW AU - Craig, S AU - Waterhouse, PM PY - 1997/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/9132064 ER -