Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans. Export

@article{citeulike:2063882, abstract = {Recently, a set of 766 genes that are enriched in the ovary as compared to the soma was identified by microarray analysis [1]. Here, we report a functional analysis of 98\% of these genes by RNA interference (RNAi). Over half the genes tested showed at least one detectable phenotype, most commonly embryonic lethality, consistent with the expectation that ovary transcripts would be enriched for genes that are essential in basic cellular and developmental processes. We find that essential genes are more likely to be conserved and to be highly expressed in the ovary. We extend previous observations and find that fewer than the expected number of ovary-expressed essential genes are present on the X chromosome. We characterized early embryonic defects for 161 genes and used time-lapse microscopy to systematically describe the defects for each gene in terms of 47 RNAi-associated phenotypes. In this paper, we discuss the use of these data to group genes into "phenoclusters"; in the accompanying paper, we use these data as one component in the integration of different types of large-scale functional analyses. We find that phenoclusters correlate well with sequence-based functional predictions and thus may be useful in predicting functions of uncharacterized genes.}, address = {Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA. fp1@nyu.edu}, author = {Piano, F. and Schetter, A. J. and Morton, D. G. and Gunsalus, K. C. and Reinke, V. and Kim, S. K. and Kemphues, K. J.}, citeulike-article-id = {2063882}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12445391}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12445391}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00005599}, day = {19}, issn = {0960-9822}, journal = {Curr Biol}, keywords = {phenotype\_review}, month = {November}, number = {22}, pages = {1959--1964}, posted-at = {2008-06-16 21:40:08}, priority = {2}, title = {Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12445391}, volume = {12}, year = {2002} }

TY - JOUR ID - citeulike:2063882 L3 - citeulike-article-id:2063882 N2 - Recently, a set of 766 genes that are enriched in the ovary as compared to the soma was identified by microarray analysis [1]. Here, we report a functional analysis of 98% of these genes by RNA interference (RNAi). Over half the genes tested showed at least one detectable phenotype, most commonly embryonic lethality, consistent with the expectation that ovary transcripts would be enriched for genes that are essential in basic cellular and developmental processes. We find that essential genes are more likely to be conserved and to be highly expressed in the ovary. We extend previous observations and find that fewer than the expected number of ovary-expressed essential genes are present on the X chromosome. We characterized early embryonic defects for 161 genes and used time-lapse microscopy to systematically describe the defects for each gene in terms of 47 RNAi-associated phenotypes. In this paper, we discuss the use of these data to group genes into "phenoclusters"; in the accompanying paper, we use these data as one component in the integration of different types of large-scale functional analyses. We find that phenoclusters correlate well with sequence-based functional predictions and thus may be useful in predicting functions of uncharacterized genes. IS - 22 JF - Curr Biol SN - 0960-9822 AD - Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA. fp1@nyu.edu EP - 1964 TI - Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans. VL - 12 SP - 1959 KW - phenotype_review AU - Piano, F AU - Schetter, AJ AU - Morton, DG AU - Gunsalus, KC AU - Reinke, V AU - Kim, SK AU - Kemphues, KJ PY - 2002/11/19/ UR - http://view.ncbi.nlm.nih.gov/pubmed/12445391 ER -