Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli. Export

@article{citeulike:3435792, abstract = {The copper stimulon in Escherichia coli consists of four regulons, the CueR-, CusS/CusR-, CpxA/CpxR-, and YedV/YedW regulons. E. coli mutants defective in cpxRA showed higher sensitivity to copper than the wild type. A total of 15 promoters were found to be induced in E. coli culture upon exposure to copper in a CpxA/CpxR-dependent manner. After gel-shift and DNase I foot-printing analyses, a conserved tandem repeat of pentanucleotide sequence, GTAAA(N)(4-8)GTAAA, with a conserved A of 4-bp upstream of each pentamer, was identified to be the CpxR-binding site. The difference in the orientation and location of the CpxR box is discussed with respect to the regulation mechanism among CpxR-regulon genes.}, author = {Yamamoto, K. and Ishihama, A.}, citeulike-article-id = {3435792}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/16861804}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=16861804}, issn = {0916-8451}, journal = {Bioscience, biotechnology, and biochemistry}, keywords = {bacteria, feedback, gene\_regulation, signaling, two-component\_system}, month = {July}, number = {7}, pages = {1688--1695}, posted-at = {2008-10-21 18:58:36}, priority = {2}, title = {Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/16861804}, volume = {70}, year = {2006} }

TY - JOUR ID - citeulike:3435792 L3 - citeulike-article-id:3435792 N2 - The copper stimulon in Escherichia coli consists of four regulons, the CueR-, CusS/CusR-, CpxA/CpxR-, and YedV/YedW regulons. E. coli mutants defective in cpxRA showed higher sensitivity to copper than the wild type. A total of 15 promoters were found to be induced in E. coli culture upon exposure to copper in a CpxA/CpxR-dependent manner. After gel-shift and DNase I foot-printing analyses, a conserved tandem repeat of pentanucleotide sequence, GTAAA(N)(4-8)GTAAA, with a conserved A of 4-bp upstream of each pentamer, was identified to be the CpxR-binding site. The difference in the orientation and location of the CpxR box is discussed with respect to the regulation mechanism among CpxR-regulon genes. IS - 7 JF - Bioscience, biotechnology, and biochemistry SN - 0916-8451 EP - 1695 TI - Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli. VL - 70 SP - 1688 KW - bacteria KW - feedback KW - gene_regulation KW - signaling KW - two-component_system AU - Yamamoto, K AU - Ishihama, A PY - 2006/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/16861804 ER -