Enhanced Macrophage Activity in Granulomatous Lesions of Immune Mice Challenged with Mycobacterium tuberculosis Export

@article{citeulike:3758061, abstract = {In this study, we evaluated the cellular influx and cytokine environment in the lungs of mice made immune by prior vaccination with Mycobacterium bovis bacillus Calmette-Guerin compared with control mice after infection with Mycobacterium tuberculosis to characterize composition of protective lesions in the lungs. Immune mice controlled the growth of the M. tuberculosis challenge more efficiently than control mice. In immune animals, granulomatous lesions were smaller and had a more lymphocytic core, less foamy cells, less parenchymal inflammation, and slower progression of lung pathology than in lungs of control mice. During the chronic stage of the infection, the bacterial load in the lungs of immune mice remained at a level 10 times lower than control mice, and this was associated with reduced numbers of CD4P+P and CD8P+P T cells, and the lower expression of protective (IL-12, IFN-{gamma}), inflammatory (TNF-{alpha}), immunoregulatory (GM-CSF), and immunosuppressive (IL-10) cytokines. The immune mice had higher numbers of CD11b-CD11chighDEC-205low alveolar macrophages, but lower numbers of CD11b+CD11chighDEC-205high dendritic cells, with the latter expressing significantly lower levels of the antiapoptotic marker TNFR-associated factor-1. Moreover, during the early stage of chronic infection, lung dendritic cells from immune mice expressed higher levels of MHC class II and CD40 molecules than similar cells from control mice. These results indicate that while a chronic disease state is the eventual outcome in both control and immune mice infected with M. tuberculosis by aerosol exposure, immune mice develop a protective granulomatous lesion by increasing macrophage numbers and reduced expression of protective and inflammatory cytokines.}, author = {Ordway, Diane and Harton, Marisa and Henao-Tamayo, Marcela and Montoya, Rose and Orme, Ian M. and Gonzalez-Juarrero, Mercedes}, citeulike-article-id = {3758061}, citeulike-linkout-0 = {http://www.jimmunol.org/cgi/content/abstract/176/8/4931}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16585589}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16585589}, day = {15}, journal = {J Immunol}, keywords = {granuloma, macrophage, mouse, mycobacterium\_tuberculosis}, month = {April}, number = {8}, pages = {4931--4939}, posted-at = {2008-12-08 22:00:14}, priority = {0}, title = {Enhanced Macrophage Activity in Granulomatous Lesions of Immune Mice Challenged with Mycobacterium tuberculosis}, url = {http://www.jimmunol.org/cgi/content/abstract/176/8/4931}, volume = {176}, year = {2006} }

TY - JOUR ID - citeulike:3758061 L3 - citeulike-article-id:3758061 N2 - In this study, we evaluated the cellular influx and cytokine environment in the lungs of mice made immune by prior vaccination with Mycobacterium bovis bacillus Calmette-Guerin compared with control mice after infection with Mycobacterium tuberculosis to characterize composition of protective lesions in the lungs. Immune mice controlled the growth of the M. tuberculosis challenge more efficiently than control mice. In immune animals, granulomatous lesions were smaller and had a more lymphocytic core, less foamy cells, less parenchymal inflammation, and slower progression of lung pathology than in lungs of control mice. During the chronic stage of the infection, the bacterial load in the lungs of immune mice remained at a level 10 times lower than control mice, and this was associated with reduced numbers of CD4P+P and CD8P+P T cells, and the lower expression of protective (IL-12, IFN-{gamma}), inflammatory (TNF-{alpha}), immunoregulatory (GM-CSF), and immunosuppressive (IL-10) cytokines. The immune mice had higher numbers of CD11b-CD11chighDEC-205low alveolar macrophages, but lower numbers of CD11b+CD11chighDEC-205high dendritic cells, with the latter expressing significantly lower levels of the antiapoptotic marker TNFR-associated factor-1. Moreover, during the early stage of chronic infection, lung dendritic cells from immune mice expressed higher levels of MHC class II and CD40 molecules than similar cells from control mice. These results indicate that while a chronic disease state is the eventual outcome in both control and immune mice infected with M. tuberculosis by aerosol exposure, immune mice develop a protective granulomatous lesion by increasing macrophage numbers and reduced expression of protective and inflammatory cytokines. IS - 8 JF - J Immunol EP - 4939 TI - Enhanced Macrophage Activity in Granulomatous Lesions of Immune Mice Challenged with Mycobacterium tuberculosis VL - 176 SP - 4931 KW - granuloma KW - macrophage KW - mouse KW - mycobacterium_tuberculosis AU - Ordway, Diane AU - Harton, Marisa AU - Henao-Tamayo, Marcela AU - Montoya, Rose AU - Orme, Ian AU - Gonzalez-Juarrero, Mercedes PY - 2006/04/15/ UR - http://www.jimmunol.org/cgi/content/abstract/176/8/4931 ER -