Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Export

@article{citeulike:4279578, abstract = {To define the mechanisms that coordinate early embryonic development and metabolism, we have examined the response of zebrafish embryos to anoxia before the midblastula transition. Our findings reveal that anoxic pre-midblastula transition embryos slow the cell cycle, arrest before the midblastula transition and can recover normally if restored to a normoxic environment. Analyses of respiratory rates reveal that pre-midblastula transition embryos are less reliant on oxidative phosphorylation than older embryos. Interestingly, arrest in anoxia occurs despite inhibition of zygotic transcription, revealing a central role for maternal factors in the response to energy limitation. Consistent with this concept, we demonstrate that the posttranslational energy-sensing AMP-activated protein kinase pathway is activated in anoxia in pre-midblastula transition embryos. Taken together, these findings demonstrate a maternal program capable of coordinating developmental rate and metabolism in the absence of transcription-based pathways or cell cycle checkpoints.}, author = {Mendelsohn, B. A. and Gitlin, J. D.}, citeulike-article-id = {4279578}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/dvdy.21584}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18521947}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18521947}, doi = {10.1002/dvdy.21584}, issn = {1058-8388}, journal = {Developmental dynamics : an official publication of the American Association of Anatomists}, keywords = {maternal, mbt, rna}, month = {July}, number = {7}, pages = {1789--1798}, posted-at = {2009-04-06 17:12:49}, priority = {2}, title = {Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo.}, url = {http://dx.doi.org/10.1002/dvdy.21584}, volume = {237}, year = {2008} }

TY - JOUR ID - citeulike:4279578 L3 - citeulike-article-id:4279578 N2 - To define the mechanisms that coordinate early embryonic development and metabolism, we have examined the response of zebrafish embryos to anoxia before the midblastula transition. Our findings reveal that anoxic pre-midblastula transition embryos slow the cell cycle, arrest before the midblastula transition and can recover normally if restored to a normoxic environment. Analyses of respiratory rates reveal that pre-midblastula transition embryos are less reliant on oxidative phosphorylation than older embryos. Interestingly, arrest in anoxia occurs despite inhibition of zygotic transcription, revealing a central role for maternal factors in the response to energy limitation. Consistent with this concept, we demonstrate that the posttranslational energy-sensing AMP-activated protein kinase pathway is activated in anoxia in pre-midblastula transition embryos. Taken together, these findings demonstrate a maternal program capable of coordinating developmental rate and metabolism in the absence of transcription-based pathways or cell cycle checkpoints. IS - 7 JF - Developmental dynamics : an official publication of the American Association of Anatomists SN - 1058-8388 EP - 1798 TI - Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. VL - 237 SP - 1789 KW - maternal KW - mbt KW - rna AU - Mendelsohn, BA AU - Gitlin, JD PY - 2008/07// UR - http://dx.doi.org/10.1002/dvdy.21584 ER -