Cap-Independent Translation Is Required for Starvation-Induced Differentiation in Yeast Export

@article{citeulike:2328004, abstract = {Cellular internal ribosome entry sites (IRESs) are untranslated segments of mRNA transcripts thought to initiate protein synthesis in response to environmental stresses that prevent canonical 5' capdependent translation. Although numerous cellular mRNAs are proposed to have IRESs, none has a demonstrated physiological function or molecular mechanism. Here we show that seven yeast genes required for invasive growth, a developmental pathway induced by nutrient limitation, contain potent IRESs that require the initiation factor eIF4G for cap-independent translation. In contrast to the RNA structure-based activity of viral IRESs, we show that an unstructured A-rich element mediates internal initiation via recruitment of the poly(A) binding protein (Pab1) to the 5' untranslated region (UTR) of invasive growth messages. A 5'UTR mutation that impairs IRES activity compromises invasive growth, which indicates that cap-independent translation is required for physiological adaptation to stress. 10.1126/science.1144467}, author = {Gilbert, Wendy V. and Zhou, Kaihong and Butler, Tamira K. and Doudna, Jennifer A.}, citeulike-article-id = {2328004}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1144467}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/317/5842/1224}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17761883}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17761883}, day = {31}, doi = {10.1126/science.1144467}, journal = {Science}, keywords = {cellular, developmental, genes, growth, initiation, ires, mechanism, molecular, mrna, mutation, pab, pathway, polya, protein, ribosome, rna, stress, structure, synthesis, translation, untranslated, utr, viral, yeast}, month = {August}, number = {5842}, pages = {1224--1227}, posted-at = {2008-04-02 16:23:19}, priority = {0}, title = {Cap-Independent Translation Is Required for Starvation-Induced Differentiation in Yeast}, url = {http://dx.doi.org/10.1126/science.1144467}, volume = {317}, year = {2007} }

TY - JOUR ID - citeulike:2328004 L3 - citeulike-article-id:2328004 N2 - Cellular internal ribosome entry sites (IRESs) are untranslated segments of mRNA transcripts thought to initiate protein synthesis in response to environmental stresses that prevent canonical 5' capdependent translation. Although numerous cellular mRNAs are proposed to have IRESs, none has a demonstrated physiological function or molecular mechanism. Here we show that seven yeast genes required for invasive growth, a developmental pathway induced by nutrient limitation, contain potent IRESs that require the initiation factor eIF4G for cap-independent translation. In contrast to the RNA structure-based activity of viral IRESs, we show that an unstructured A-rich element mediates internal initiation via recruitment of the poly(A) binding protein (Pab1) to the 5' untranslated region (UTR) of invasive growth messages. A 5'UTR mutation that impairs IRES activity compromises invasive growth, which indicates that cap-independent translation is required for physiological adaptation to stress. 10.1126/science.1144467 IS - 5842 JF - Science EP - 1227 TI - Cap-Independent Translation Is Required for Starvation-Induced Differentiation in Yeast VL - 317 SP - 1224 KW - cellular KW - developmental KW - genes KW - growth KW - initiation KW - ires KW - mechanism KW - molecular KW - mrna KW - mutation KW - pab KW - pathway KW - polya KW - protein KW - ribosome KW - rna KW - stress KW - structure KW - synthesis KW - translation KW - untranslated KW - utr KW - viral KW - yeast AU - Gilbert, Wendy AU - Zhou, Kaihong AU - Butler, Tamira AU - Doudna, Jennifer PY - 2007/08/31/ UR - http://dx.doi.org/10.1126/science.1144467 ER -