A bona fide La protein is required for embryogenesis in Arabidopsis thaliana Export

@article{citeulike:2642531, abstract = {Searches in the Arabidopsis thaliana genome using the La motif as query revealed the presence of eight La or La-like proteins. Using structural and phylogenetic criteria, we identified two putative genuine La proteins (At32 and At79) and showed that both are expressed throughout plant development but at different levels and under different regulatory conditions. At32, but not At79, restores Saccharomyces cerevisiae La nuclear functions in non-coding RNAs biogenesis and is able to bind to plant 3'-UUU-OH RNAs. We conclude that these La nuclear functions are conserved in Arabidopsis and supported by At32, which we renamed as AtLa1. Consistently, AtLa1 is predominantly localized to the plant nucleoplasm and was also detected in the nucleolar cavity. The inactivation of AtLa1 in Arabidopsis leads to an embryonic-lethal phenotype with deficient embryos arrested at early globular stage of development. In addition, mutant embryonic cells display a nucleolar hypertrophy suggesting that AtLa1 is required for normal ribosome biogenesis. The identification of two distantly related proteins with all structural characteristics of genuine La proteins suggests that these factors evolved to a certain level of specialization in plants. This unprecedented situation provides a unique opportunity to dissect the very different aspects of this crucial cellular activity. 10.1093/nar/gkm200}, author = {Fleurdepine, Sophie and Deragon, Jean-Marc and Devic, Martine and Guilleminot, Jocelyne and Bousquet-Antonelli, Cecile}, citeulike-article-id = {2642531}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkm200}, citeulike-linkout-1 = {http://nar.oxfordjournals.org/cgi/content/abstract/35/10/3306}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17459889}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17459889}, day = {11}, doi = {10.1093/nar/gkm200}, journal = {Nucl. Acids Res.}, keywords = {arabidopsis, biogenesis, cerevisiae, development, embryogenesis, embryonic, genome, la, lethal, motif, mutant, nuclear, nucleolar, nucleoplasm, phenotype, phylogenetic, plant, protein, ribosome, rna, saccharomyces, thaliana}, month = {May}, number = {10}, pages = {3306--3321}, posted-at = {2008-04-08 19:44:53}, priority = {0}, title = {A bona fide La protein is required for embryogenesis in Arabidopsis thaliana}, url = {http://dx.doi.org/10.1093/nar/gkm200}, volume = {35}, year = {2007} }

TY - JOUR ID - citeulike:2642531 L3 - citeulike-article-id:2642531 N2 - Searches in the Arabidopsis thaliana genome using the La motif as query revealed the presence of eight La or La-like proteins. Using structural and phylogenetic criteria, we identified two putative genuine La proteins (At32 and At79) and showed that both are expressed throughout plant development but at different levels and under different regulatory conditions. At32, but not At79, restores Saccharomyces cerevisiae La nuclear functions in non-coding RNAs biogenesis and is able to bind to plant 3'-UUU-OH RNAs. We conclude that these La nuclear functions are conserved in Arabidopsis and supported by At32, which we renamed as AtLa1. Consistently, AtLa1 is predominantly localized to the plant nucleoplasm and was also detected in the nucleolar cavity. The inactivation of AtLa1 in Arabidopsis leads to an embryonic-lethal phenotype with deficient embryos arrested at early globular stage of development. In addition, mutant embryonic cells display a nucleolar hypertrophy suggesting that AtLa1 is required for normal ribosome biogenesis. The identification of two distantly related proteins with all structural characteristics of genuine La proteins suggests that these factors evolved to a certain level of specialization in plants. This unprecedented situation provides a unique opportunity to dissect the very different aspects of this crucial cellular activity. 10.1093/nar/gkm200 IS - 10 JF - Nucl. Acids Res. EP - 3321 TI - A bona fide La protein is required for embryogenesis in Arabidopsis thaliana VL - 35 SP - 3306 KW - arabidopsis KW - biogenesis KW - cerevisiae KW - development KW - embryogenesis KW - embryonic KW - genome KW - la KW - lethal KW - motif KW - mutant KW - nuclear KW - nucleolar KW - nucleoplasm KW - phenotype KW - phylogenetic KW - plant KW - protein KW - ribosome KW - rna KW - saccharomyces KW - thaliana AU - Fleurdepine, Sophie AU - Deragon, Jean-Marc AU - Devic, Martine AU - Guilleminot, Jocelyne AU - Bousquet-Antonelli, Cecile PY - 2007/05/11/ UR - http://dx.doi.org/10.1093/nar/gkm200 ER -