MicroRNA-10a Binds the 5'UTR of Ribosomal Protein mRNAs and Enhances Their Translation

@article{citeulike:2847256, abstract = {Summary MicroRNAs (miRNAs) are small RNAs that function as posttranscriptional regulators of gene expression. miRNAs affect a variety of signaling pathways, and impaired miRNA regulation may contribute to the development of cancer and other diseases. Here we show that miRNA miR-10a interacts with the 5' untranslated region of mRNAs encoding ribosomal proteins to enhance their translation. miR-10a alleviates translational repression of the ribosomal protein mRNAs during amino acid starvation and is required for their translational induction following anisomycin treatment or overexpression of RAS. We show that miR-10a binds immediately downstream of the regulatory 5'TOP motif and that the 5'TOP regulatory complex and miR-10a are functionally interconnected. The results show that miR-10a may positively control global protein synthesis via the stimulation of ribosomal protein mRNA translation and ribosome biogenesis and hereby affect the ability of cells to undergo transformation.}, author = {{\O}rom, Ulf A. and Nielsen, Finn C. and Lund, Anders H.}, citeulike-article-id = {2847256}, doi = {10.1016/j.molcel.2008.05.001}, journal = {Molecular Cell}, keywords = {mirna, mrna, ribosome, top, translation}, month = {May}, number = {4}, pages = {460--471}, posted-at = {2008-09-12 22:24:39}, priority = {4}, title = {MicroRNA-10a Binds the 5'UTR of Ribosomal Protein mRNAs and Enhances Their Translation}, url = {http://dx.doi.org/10.1016/j.molcel.2008.05.001}, volume = {30}, year = {2008} }

TY - JOUR ID - citeulike:2847256 L3 - citeulike-article-id:2847256 N2 - Summary MicroRNAs (miRNAs) are small RNAs that function as posttranscriptional regulators of gene expression. miRNAs affect a variety of signaling pathways, and impaired miRNA regulation may contribute to the development of cancer and other diseases. Here we show that miRNA miR-10a interacts with the 5' untranslated region of mRNAs encoding ribosomal proteins to enhance their translation. miR-10a alleviates translational repression of the ribosomal protein mRNAs during amino acid starvation and is required for their translational induction following anisomycin treatment or overexpression of RAS. We show that miR-10a binds immediately downstream of the regulatory 5'TOP motif and that the 5'TOP regulatory complex and miR-10a are functionally interconnected. The results show that miR-10a may positively control global protein synthesis via the stimulation of ribosomal protein mRNA translation and ribosome biogenesis and hereby affect the ability of cells to undergo transformation. IS - 4 JF - Molecular Cell EP - 471 TI - MicroRNA-10a Binds the 5'UTR of Ribosomal Protein mRNAs and Enhances Their Translation VL - 30 SP - 460 KW - mirna KW - mrna KW - ribosome KW - top KW - translation AU - Ørom, Ulf AU - Nielsen, Finn AU - Lund, Anders PY - 2008/05/23/ UR - http://dx.doi.org/10.1016/j.molcel.2008.05.001 ER -