Analysis of mouse embryonic patterning and morphogenesis by forward genetics. Export

@article{citeulike:167801, abstract = {This contribution is part of the special series of Inaugural Articles by members of the National Academy of Sciences elected on April 20, 2004. Many aspects of the genetic control of mammalian embryogenesis cannot be extrapolated from other animals. Taking a forward genetic approach, we have induced recessive mutations by treatment of mice with ethylnitrosourea and have identified 43 mutations that affect early morphogenesis and patterning, including 38 genes that have not been studied previously. The molecular lesions responsible for 14 mutations were identified, including mutations in nine genes that had not been characterized previously. Some mutations affect vertebrate-specific components of conserved signaling pathways; for example, at least five mutations affect previously uncharacterized regulators of the Sonic hedgehog (Shh) pathway. Approximately half of all of the mutations affect the initial establishment of the body plan, and several of these produce phenotypes that have not been described previously. A large fraction of the genes identified affect cell migration, cellular organization, and cell structure. The findings indicate that phenotype-based genetic screens provide a direct and unbiased method to identify essential regulators of mammalian development.}, address = {Developmental Biology Program, Sloan-Kettering Institute, 1275 York Avenue, New York, NY 10021; Neuroscience Program and Molecular and Cell Biology Program, Weill Graduate School of Medical Sciences, Cornell University, 445 East 69th Street, New York, NY 10021.}, author = {Garc\'{\i}a-Garc\'{\i}a, Mar\'{\i}a J J. and Eggenschwiler, Jonathan T T. and Caspary, Tamara and Alcorn, Heather L L. and Wyler, Michael R R. and Huangfu, Danwei and Rakeman, Andrew S S. and Lee, Jeffrey D D. and Feinberg, Evan H H. and Timmer, John R R. and Anderson, Kathryn V V.}, citeulike-article-id = {167801}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0501071102}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15755804}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15755804}, day = {8}, doi = {10.1073/pnas.0501071102}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {development, enu, mouse}, month = {March}, posted-at = {2005-04-23 00:11:56}, priority = {0}, title = {Analysis of mouse embryonic patterning and morphogenesis by forward genetics.}, url = {http://dx.doi.org/10.1073/pnas.0501071102}, year = {2005} }

TY - JOUR ID - citeulike:167801 L3 - citeulike-article-id:167801 N2 - This contribution is part of the special series of Inaugural Articles by members of the National Academy of Sciences elected on April 20, 2004. Many aspects of the genetic control of mammalian embryogenesis cannot be extrapolated from other animals. Taking a forward genetic approach, we have induced recessive mutations by treatment of mice with ethylnitrosourea and have identified 43 mutations that affect early morphogenesis and patterning, including 38 genes that have not been studied previously. The molecular lesions responsible for 14 mutations were identified, including mutations in nine genes that had not been characterized previously. Some mutations affect vertebrate-specific components of conserved signaling pathways; for example, at least five mutations affect previously uncharacterized regulators of the Sonic hedgehog (Shh) pathway. Approximately half of all of the mutations affect the initial establishment of the body plan, and several of these produce phenotypes that have not been described previously. A large fraction of the genes identified affect cell migration, cellular organization, and cell structure. The findings indicate that phenotype-based genetic screens provide a direct and unbiased method to identify essential regulators of mammalian development. JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Developmental Biology Program, Sloan-Kettering Institute, 1275 York Avenue, New York, NY 10021; Neuroscience Program and Molecular and Cell Biology Program, Weill Graduate School of Medical Sciences, Cornell University, 445 East 69th Street, New York, NY 10021. TI - Analysis of mouse embryonic patterning and morphogenesis by forward genetics. KW - development KW - enu KW - mouse AU - García-García, María J AU - Eggenschwiler, Jonathan T AU - Caspary, Tamara AU - Alcorn, Heather L AU - Wyler, Michael R AU - Huangfu, Danwei AU - Rakeman, Andrew S AU - Lee, Jeffrey D AU - Feinberg, Evan H AU - Timmer, John R AU - Anderson, Kathryn V PY - 2005/03/08/ UR - http://dx.doi.org/10.1073/pnas.0501071102 ER -