Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella. Export

@article{citeulike:171259, abstract = {Proteins necessary for maintenance and function of eukaryotic flagella are synthesized in the cell body. Transport of the inner dynein arm subunit p28(IDA4) in Chlamydomonas flagella requires the activity of the kinesin KHP1(FLA10), a protein inactive at restrictive temperature in fla10, a temperature-dependent mutant of flagellar assembly. To identify other molecules involved in active transport of inner dynein arms within flagella we searched for polypeptides of the cytoplasmic matrix of flagella that fulfill two conditions: they bind to p28 and require the activity of KHP1 to be present in flagella. We found that the cytoplasmic matrix of flagella contains a previously unidentified "17S" complex of at least 13 polypeptides that in part is associated with p28. The 17S complex is present at permissive but not at restrictive temperature in fla10 flagella. It also turns over in the cytoplasmic matrix more frequently than dynein arms within the axoneme. This evidence suggests that the 17S complex transports precursors of inner dynein arms within flagella.}, address = {Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, 1 Gustave Levy Place, Box 1007, New York, NY 10029, USA.}, author = {Piperno, G. and Mead, K.}, citeulike-article-id = {171259}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9114011}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9114011}, day = {29}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {chlamy, ift}, month = {April}, number = {9}, pages = {4457--4462}, posted-at = {2005-04-25 21:59:50}, priority = {0}, title = {Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9114011}, volume = {94}, year = {1997} }

TY - JOUR ID - citeulike:171259 L3 - citeulike-article-id:171259 N2 - Proteins necessary for maintenance and function of eukaryotic flagella are synthesized in the cell body. Transport of the inner dynein arm subunit p28(IDA4) in Chlamydomonas flagella requires the activity of the kinesin KHP1(FLA10), a protein inactive at restrictive temperature in fla10, a temperature-dependent mutant of flagellar assembly. To identify other molecules involved in active transport of inner dynein arms within flagella we searched for polypeptides of the cytoplasmic matrix of flagella that fulfill two conditions: they bind to p28 and require the activity of KHP1 to be present in flagella. We found that the cytoplasmic matrix of flagella contains a previously unidentified "17S" complex of at least 13 polypeptides that in part is associated with p28. The 17S complex is present at permissive but not at restrictive temperature in fla10 flagella. It also turns over in the cytoplasmic matrix more frequently than dynein arms within the axoneme. This evidence suggests that the 17S complex transports precursors of inner dynein arms within flagella. IS - 9 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, 1 Gustave Levy Place, Box 1007, New York, NY 10029, USA. EP - 4462 TI - Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella. VL - 94 SP - 4457 KW - chlamy KW - ift AU - Piperno, G AU - Mead, K PY - 1997/04/29/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9114011 ER -