The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova. Export

@article{citeulike:179600, abstract = {The gastrulating vertebrate embryo develops three germlayers: ectoderm, mesoderm, and endoderm. Zebrafish endoderm differentiation starts with the activation of sox17 by casanova (cas). We report that spg (pou2/Oct4) is essential for endoderm formation. Embryos devoid of maternal and zygotic spg function (MZspg) lack endodermal precursors. Cell transplantations show that spg acts in early endodermal precursors, and cas mRNA-injection into MZspg embryos does not restore endoderm development. spg and cas together are both necessary and sufficient to activate endoderm development, and stimulate expression of a sox17 promoter-luciferase reporter. Endoderm and mesoderm derive from a common origin, mesendoderm. We propose that Spg and Cas commit mesendodermal precursors to an endodermal fate. The joint control of endoderm formation by spg and cas suggests that the endodermal germlayer may be a tissue unit with distinct genetic control, thus adding genetic support to the germlayer concept in metazoan development.}, address = {MPI of Molecular Cell Biology and Genetics, and Department of Genetics, TU Dresden, Pfotenhauerstrasse 108, D-01309 Dresden, Germany.}, author = {Reim, G. and Mizoguchi, T. and Stainier, D. Y. and Kikuchi, Y. and Brand, M.}, citeulike-article-id = {179600}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/14723850}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=14723850}, issn = {1534-5807}, journal = {Dev Cell}, keywords = {cas, endoderm, oct4, sox17}, month = {January}, number = {1}, pages = {91--101}, posted-at = {2005-05-03 17:37:16}, priority = {2}, title = {The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/14723850}, volume = {6}, year = {2004} }

TY - JOUR ID - citeulike:179600 L3 - citeulike-article-id:179600 N2 - The gastrulating vertebrate embryo develops three germlayers: ectoderm, mesoderm, and endoderm. Zebrafish endoderm differentiation starts with the activation of sox17 by casanova (cas). We report that spg (pou2/Oct4) is essential for endoderm formation. Embryos devoid of maternal and zygotic spg function (MZspg) lack endodermal precursors. Cell transplantations show that spg acts in early endodermal precursors, and cas mRNA-injection into MZspg embryos does not restore endoderm development. spg and cas together are both necessary and sufficient to activate endoderm development, and stimulate expression of a sox17 promoter-luciferase reporter. Endoderm and mesoderm derive from a common origin, mesendoderm. We propose that Spg and Cas commit mesendodermal precursors to an endodermal fate. The joint control of endoderm formation by spg and cas suggests that the endodermal germlayer may be a tissue unit with distinct genetic control, thus adding genetic support to the germlayer concept in metazoan development. IS - 1 JF - Dev Cell SN - 1534-5807 AD - MPI of Molecular Cell Biology and Genetics, and Department of Genetics, TU Dresden, Pfotenhauerstrasse 108, D-01309 Dresden, Germany. EP - 101 TI - The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova. VL - 6 SP - 91 KW - cas KW - endoderm KW - oct4 KW - sox17 AU - Reim, G AU - Mizoguchi, T AU - Stainier, DY AU - Kikuchi, Y AU - Brand, M PY - 2004/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/14723850 ER -