A focused and efficient genetic screening strategy in the mouse: identification of mutations that disrupt cortical development. Export

@article{citeulike:180197, abstract = {Although the mechanisms that regulate development of the cerebral cortex have begun to emerge, in large part through the analysis of mutant mice (Boncinelli et al. 2000; Molnar and Hannan 2000; Walsh and Goffinet 2000), many questions remain unanswered. To provide resources for further dissecting cortical development, we have carried out a focused screen for recessive mutations that disrupt cortical development. One aim of the screen was to identify mutants that disrupt the tangential migration of interneurons into the cortex. At the same time, we also screened for mutations that altered the growth or morphology of the cerebral cortex. We report here the identification of thirteen mutants with defects in aspects of cortical development ranging from the establishment of epithelial polarity to the invasion of thalamocortical axons. Among the collection are three novel alleles of genes for which mutant alleles had already been used to explore forebrain development, and four mutants with defects in interneuron migration. The mutants that we describe here will aid in deciphering the molecules and mechanisms that regulate cortical development. Our results also highlight the utility of focused screens in the mouse, in addition to the large-scale and broadly targeted screens that are being carried out at mutagenesis centers.}, address = {Department of Neurology and the Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, USA.}, author = {Zarbalis, K. and May, S. R. and Shen, Y. and Ekker, M. and Rubenstein, J. L. and Peterson, A. S.}, citeulike-article-id = {180197}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pbio.0020219}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15314648}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15314648}, doi = {10.1371/journal.pbio.0020219}, issn = {1545-7885}, journal = {PLoS Biol}, keywords = {brain, enu, mouse, rfx4}, month = {August}, number = {8}, posted-at = {2005-05-04 19:02:49}, priority = {2}, title = {A focused and efficient genetic screening strategy in the mouse: identification of mutations that disrupt cortical development.}, url = {http://dx.doi.org/10.1371/journal.pbio.0020219}, volume = {2}, year = {2004} }

TY - JOUR ID - citeulike:180197 L3 - citeulike-article-id:180197 N2 - Although the mechanisms that regulate development of the cerebral cortex have begun to emerge, in large part through the analysis of mutant mice (Boncinelli et al. 2000; Molnar and Hannan 2000; Walsh and Goffinet 2000), many questions remain unanswered. To provide resources for further dissecting cortical development, we have carried out a focused screen for recessive mutations that disrupt cortical development. One aim of the screen was to identify mutants that disrupt the tangential migration of interneurons into the cortex. At the same time, we also screened for mutations that altered the growth or morphology of the cerebral cortex. We report here the identification of thirteen mutants with defects in aspects of cortical development ranging from the establishment of epithelial polarity to the invasion of thalamocortical axons. Among the collection are three novel alleles of genes for which mutant alleles had already been used to explore forebrain development, and four mutants with defects in interneuron migration. The mutants that we describe here will aid in deciphering the molecules and mechanisms that regulate cortical development. Our results also highlight the utility of focused screens in the mouse, in addition to the large-scale and broadly targeted screens that are being carried out at mutagenesis centers. IS - 8 JF - PLoS Biol SN - 1545-7885 AD - Department of Neurology and the Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, USA. TI - A focused and efficient genetic screening strategy in the mouse: identification of mutations that disrupt cortical development. VL - 2 KW - brain KW - enu KW - mouse KW - rfx4 AU - Zarbalis, K AU - May, SR AU - Shen, Y AU - Ekker, M AU - Rubenstein, JL AU - Peterson, AS PY - 2004/08// UR - http://dx.doi.org/10.1371/journal.pbio.0020219 ER -