An acylatable residue of Hedgehog is differentially required in Drosophila and mouse limb development. Export

@article{citeulike:201630, abstract = {The Drosophila Hedgehog protein and its vertebrate counterpart Sonic hedgehog are required for a wide variety of patterning events throughout development. Hedgehog proteins are secreted from cells and undergo autocatalytic cleavage and cholesterol modification to produce a mature signaling domain. This domain of Sonic hedgehog has recently been shown to acquire an N-terminal acyl group in cell culture. We have investigated the in vivo role that such acylation might play in appendage patterning in mouse and Drosophila; in both species Hedgehog proteins define a posterior domain of the limb or wing. A mutant form of Sonic hedgehog that cannot undergo acylation retains significant ability to repattern the mouse limb. However, the corresponding mutation in Drosophila Hedgehog renders it inactive in vivo, although it is normally processed. Furthermore, overexpression of the mutant form has dominant negative effects on Hedgehog signaling. These data suggest that the importance of the N-terminal cysteine of mature Hedgehog in patterning appendages differs between species.}, address = {Skirball Institute Developmental Genetics Program, Department of Cell Biology, NYU School of Medicine, 540 First Avenue, New York, New York 10016, USA.}, author = {Lee, J. D. and Kraus, P. and Gaiano, N. and Nery, S. and Kohtz, J. and Fishell, G. and Loomis, C. A. and Treisman, J. E.}, citeulike-article-id = {201630}, citeulike-linkout-0 = {http://dx.doi.org/10.1006/dbio.2001.0218}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11319862}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11319862}, day = {1}, doi = {10.1006/dbio.2001.0218}, issn = {0012-1606}, journal = {Dev Biol}, keywords = {drosophila, hedgehog, mouse}, month = {May}, number = {1}, pages = {122--136}, posted-at = {2005-05-16 21:14:08}, priority = {2}, title = {An acylatable residue of Hedgehog is differentially required in Drosophila and mouse limb development.}, url = {http://dx.doi.org/10.1006/dbio.2001.0218}, volume = {233}, year = {2001} }

TY - JOUR ID - citeulike:201630 L3 - citeulike-article-id:201630 N2 - The Drosophila Hedgehog protein and its vertebrate counterpart Sonic hedgehog are required for a wide variety of patterning events throughout development. Hedgehog proteins are secreted from cells and undergo autocatalytic cleavage and cholesterol modification to produce a mature signaling domain. This domain of Sonic hedgehog has recently been shown to acquire an N-terminal acyl group in cell culture. We have investigated the in vivo role that such acylation might play in appendage patterning in mouse and Drosophila; in both species Hedgehog proteins define a posterior domain of the limb or wing. A mutant form of Sonic hedgehog that cannot undergo acylation retains significant ability to repattern the mouse limb. However, the corresponding mutation in Drosophila Hedgehog renders it inactive in vivo, although it is normally processed. Furthermore, overexpression of the mutant form has dominant negative effects on Hedgehog signaling. These data suggest that the importance of the N-terminal cysteine of mature Hedgehog in patterning appendages differs between species. IS - 1 JF - Dev Biol SN - 0012-1606 AD - Skirball Institute Developmental Genetics Program, Department of Cell Biology, NYU School of Medicine, 540 First Avenue, New York, New York 10016, USA. EP - 136 TI - An acylatable residue of Hedgehog is differentially required in Drosophila and mouse limb development. VL - 233 SP - 122 KW - drosophila KW - hedgehog KW - mouse AU - Lee, JD AU - Kraus, P AU - Gaiano, N AU - Nery, S AU - Kohtz, J AU - Fishell, G AU - Loomis, CA AU - Treisman, JE PY - 2001/05/01/ UR - http://dx.doi.org/10.1006/dbio.2001.0218 ER -