Mechanism of Nodal Flow: A Conserved Symmetry Breaking Event in Left-Right Axis Determination Export

@article{citeulike:212884, abstract = {SummaryThe leftward flow in extraembryonic fluid is critical for the initial determination of the left-right axis of mouse embryos. It is unclear if this is a conserved mechanism among other vertebrates and how the directionality of the flow arises from the motion of cilia. In this paper, we show that rabbit and medakafish embryos also exhibit a leftward fluid flow in their ventral nodes. In all cases, primary monocilia present a clockwise rotational-like motion. Observations of defective ciliary dynamics in mutant mouse embryos support the idea that the posterior tilt of the cilia during rotational-like beating can explain the leftward fluid flow. Moreover, we show that this leftward flow may produce asymmetric distribution of exogenously introduced proteins, suggesting morphogen gradients as a subsequent mechanism of left-right axis determination. Finally, we experimentally and theoretically characterize under which conditions a morphogen gradient can arise from the flow.}, author = {Okada, Yasushi and Takeda, Sen and Tanaka, Yosuke and Belmonte, Juan-Carlos I. and Hirokawa, Nobutaka}, citeulike-article-id = {212884}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2005.04.008}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WSN-4G6KR00-M/2/30513a26652faa1aff09f1312b48348f}, day = {20}, doi = {10.1016/j.cell.2005.04.008}, journal = {Cell}, keywords = {cilia, lr, node}, month = {May}, number = {4}, pages = {633--644}, posted-at = {2005-05-27 15:03:24}, priority = {2}, title = {Mechanism of Nodal Flow: A Conserved Symmetry Breaking Event in Left-Right Axis Determination}, url = {http://dx.doi.org/10.1016/j.cell.2005.04.008}, volume = {121}, year = {2005} }

TY - JOUR ID - citeulike:212884 L3 - citeulike-article-id:212884 N2 - SummaryThe leftward flow in extraembryonic fluid is critical for the initial determination of the left-right axis of mouse embryos. It is unclear if this is a conserved mechanism among other vertebrates and how the directionality of the flow arises from the motion of cilia. In this paper, we show that rabbit and medakafish embryos also exhibit a leftward fluid flow in their ventral nodes. In all cases, primary monocilia present a clockwise rotational-like motion. Observations of defective ciliary dynamics in mutant mouse embryos support the idea that the posterior tilt of the cilia during rotational-like beating can explain the leftward fluid flow. Moreover, we show that this leftward flow may produce asymmetric distribution of exogenously introduced proteins, suggesting morphogen gradients as a subsequent mechanism of left-right axis determination. Finally, we experimentally and theoretically characterize under which conditions a morphogen gradient can arise from the flow. IS - 4 JF - Cell EP - 644 TI - Mechanism of Nodal Flow: A Conserved Symmetry Breaking Event in Left-Right Axis Determination VL - 121 SP - 633 KW - cilia KW - lr KW - node AU - Okada, Yasushi AU - Takeda, Sen AU - Tanaka, Yosuke AU - Belmonte, Juan-Carlos AU - Hirokawa, Nobutaka PY - 2005/05/20/ UR - http://dx.doi.org/10.1016/j.cell.2005.04.008 ER -