Hedgehog-regulated Costal2-kinase complexes control phosphorylation and proteolytic processing of Cubitus interruptus. Export

@article{citeulike:220810, abstract = {Hedgehog (Hh) proteins control animal development by regulating the Gli/Ci family of transcription factors. In Drosophila, Hh counteracts phosphorylation by PKA, GSK3, and CKI to prevent Cubitus interruptus (Ci) processing through unknown mechanisms. Here, we show that these kinases physically interact with the kinesin-like protein Costal2 (Cos2) to control Ci processing and that Hh inhibits such interaction. Cos2 is required for Ci phosphorylation in vivo, and Cos2-immunocomplexes (Cos2IPs) phosphorylate Ci and contain PKA, GSK3, and CKI. By using a Kinesin-Cos2 chimeric protein that carries Cos2-interacting proteins to the microtubule plus end, we demonstrated that these kinases bind Cos2 in intact cells. PKA, GSK3, and CKI directly bind the N- and C-terminal regions of Cos2, both of which are essential for Ci processing. Finally, we showed that Hh signaling inhibits Cos2-kinase complex formation. We propose that Cos2 recruits multiple kinases to efficiently phosphorylate Ci and that Hh inhibits Ci phosphorylation by specifically interfering with kinase recruitment.}, address = {Center for Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.}, author = {Zhang, W. and Zhao, Y. and Tong, C. and Wang, G. and Wang, B. and Jia, J. and Jiang, J.}, citeulike-article-id = {220810}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.devcel.2005.01.001}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15691767}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15691767}, doi = {10.1016/j.devcel.2005.01.001}, issn = {1534-5807}, journal = {Dev Cell}, keywords = {ci, cos2, drosohpila, hedgehog, phosphorylation}, month = {February}, number = {2}, pages = {267--278}, posted-at = {2005-06-06 17:35:38}, priority = {2}, title = {Hedgehog-regulated Costal2-kinase complexes control phosphorylation and proteolytic processing of Cubitus interruptus.}, url = {http://dx.doi.org/10.1016/j.devcel.2005.01.001}, volume = {8}, year = {2005} }

TY - JOUR ID - citeulike:220810 L3 - citeulike-article-id:220810 N2 - Hedgehog (Hh) proteins control animal development by regulating the Gli/Ci family of transcription factors. In Drosophila, Hh counteracts phosphorylation by PKA, GSK3, and CKI to prevent Cubitus interruptus (Ci) processing through unknown mechanisms. Here, we show that these kinases physically interact with the kinesin-like protein Costal2 (Cos2) to control Ci processing and that Hh inhibits such interaction. Cos2 is required for Ci phosphorylation in vivo, and Cos2-immunocomplexes (Cos2IPs) phosphorylate Ci and contain PKA, GSK3, and CKI. By using a Kinesin-Cos2 chimeric protein that carries Cos2-interacting proteins to the microtubule plus end, we demonstrated that these kinases bind Cos2 in intact cells. PKA, GSK3, and CKI directly bind the N- and C-terminal regions of Cos2, both of which are essential for Ci processing. Finally, we showed that Hh signaling inhibits Cos2-kinase complex formation. We propose that Cos2 recruits multiple kinases to efficiently phosphorylate Ci and that Hh inhibits Ci phosphorylation by specifically interfering with kinase recruitment. IS - 2 JF - Dev Cell SN - 1534-5807 AD - Center for Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA. EP - 278 TI - Hedgehog-regulated Costal2-kinase complexes control phosphorylation and proteolytic processing of Cubitus interruptus. VL - 8 SP - 267 KW - ci KW - cos2 KW - drosohpila KW - hedgehog KW - phosphorylation AU - Zhang, W AU - Zhao, Y AU - Tong, C AU - Wang, G AU - Wang, B AU - Jia, J AU - Jiang, J PY - 2005/02// UR - http://dx.doi.org/10.1016/j.devcel.2005.01.001 ER -