Using genomewide mutagenesis screens to identify the genes required for neural tube closure in the mouse. Export

@article{citeulike:236134, abstract = {BACKGROUND: Neural tube closure is a critical embryological process that requires the coordination of many molecular and cellular events. Only recently has the molecular basis of the cell movements that drive neural tube closure begun to be elucidated. This has been accomplished in part due to the analysis of a growing number of genetically targeted and naturally occurring mouse mutant strains that have neural tube defects (NTDs). Currently there are more than 100 genes that when mutated result in NTDs in the mouse. Yet only approximately 10\% of genes in the mouse genome have been mutated and their gross phenotype analyzed, suggesting that only a small percentage of the genes that can cause NTDs have been identified. METHODS: In order to more systematically and fully understand the genetic basis of neural tube closure and to begin to define the molecular pathways that direct this key embryonic event, our laboratories have undertaken a forward genetic screen in mice. From this we hope to gain a better understanding of the regulation of this complex morphogenic processes. CONCLUSIONS: The mouse provides a good model for human neural tube closure, and therefore the information gained from generating novel mouse models of NTDs will help to predict the genes responsible for human NTDs and provide experimental evidence for how they function. Birth Defects Research (Part A), 2005. (c) 2005 Wiley-Liss, Inc.}, address = {Developmental Biology Program, Sloan‐Kettering Institute, New York, New York.}, author = {Zohn, Irene E E. and Anderson, Kathryn V V. and Niswander, Lee}, citeulike-article-id = {236134}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/bdra.20164}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15971254}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15971254}, day = {21}, doi = {10.1002/bdra.20164}, issn = {1542-0752}, journal = {Birth Defects Res A Clin Mol Teratol}, keywords = {enu, neural-tube}, month = {June}, posted-at = {2005-06-24 15:37:31}, priority = {2}, title = {Using genomewide mutagenesis screens to identify the genes required for neural tube closure in the mouse.}, url = {http://dx.doi.org/10.1002/bdra.20164}, year = {2005} }

TY - JOUR ID - citeulike:236134 L3 - citeulike-article-id:236134 N2 - BACKGROUND: Neural tube closure is a critical embryological process that requires the coordination of many molecular and cellular events. Only recently has the molecular basis of the cell movements that drive neural tube closure begun to be elucidated. This has been accomplished in part due to the analysis of a growing number of genetically targeted and naturally occurring mouse mutant strains that have neural tube defects (NTDs). Currently there are more than 100 genes that when mutated result in NTDs in the mouse. Yet only approximately 10% of genes in the mouse genome have been mutated and their gross phenotype analyzed, suggesting that only a small percentage of the genes that can cause NTDs have been identified. METHODS: In order to more systematically and fully understand the genetic basis of neural tube closure and to begin to define the molecular pathways that direct this key embryonic event, our laboratories have undertaken a forward genetic screen in mice. From this we hope to gain a better understanding of the regulation of this complex morphogenic processes. CONCLUSIONS: The mouse provides a good model for human neural tube closure, and therefore the information gained from generating novel mouse models of NTDs will help to predict the genes responsible for human NTDs and provide experimental evidence for how they function. Birth Defects Research (Part A), 2005. (c) 2005 Wiley-Liss, Inc. JF - Birth Defects Res A Clin Mol Teratol SN - 1542-0752 AD - Developmental Biology Program, Sloan‐Kettering Institute, New York, New York. TI - Using genomewide mutagenesis screens to identify the genes required for neural tube closure in the mouse. KW - enu KW - neural-tube AU - Zohn, Irene E AU - Anderson, Kathryn V AU - Niswander, Lee PY - 2005/06/21/ UR - http://dx.doi.org/10.1002/bdra.20164 ER -