Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G Export

@article{citeulike:709355, abstract = {Alterations in endoplasmic reticulum (ER) cholesterol are fundamental for a variety of cellular processes such as the regulation of lipid homeostasis or efficient protein degradation. We show that reduced levels of cellular sterols cause a delayed ER-to-Golgi transport of the secretory cargo membrane protein ts-O45-G and a relocation to the ER of an endogenous protein cycling between the ER and the Golgi complex. Transport inhibition is characterized by a delay in the accumulation of ts-O45-G in ER-exit sites (ERES) and correlates with a reduced mobility of ts-O45-G within ER membranes. A simple mathematical model describing the kinetics of ER-exit predicts that reduced cargo loading to ERES and not the reduced mobility of ts-O45-G accounts for the delayed ER-exit and arrival at the Golgi. Consistent with this, membrane turnover of the COPII component Sec23p is delayed in sterol-depleted cells. Altogether, our results demonstrate the importance of sterol levels in COPII mediated ER-export.}, author = {Runz, Heiko and Miura, Kota and Weiss, Matthias and Pepperkok, Rainer}, citeulike-article-id = {709355}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/sj.emboj.7601205}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/7601205a}, day = {22}, doi = {10.1038/sj.emboj.7601205}, issn = {0261-4189}, journal = {The EMBO Journal}, keywords = {cholesterol, copii, er, sterol, vesicle}, month = {June}, number = {13}, pages = {2953--2965}, posted-at = {2009-11-05 20:27:29}, priority = {0}, publisher = {Nature Publishing Group}, title = {Sterols regulate {ER}-export dynamics of secretory cargo protein {ts-O45-G}}, url = {http://dx.doi.org/10.1038/sj.emboj.7601205}, volume = {25}, year = {2006} }

TY - JOUR ID - citeulike:709355 L3 - citeulike-article-id:709355 N2 - Alterations in endoplasmic reticulum (ER) cholesterol are fundamental for a variety of cellular processes such as the regulation of lipid homeostasis or efficient protein degradation. We show that reduced levels of cellular sterols cause a delayed ER-to-Golgi transport of the secretory cargo membrane protein ts-O45-G and a relocation to the ER of an endogenous protein cycling between the ER and the Golgi complex. Transport inhibition is characterized by a delay in the accumulation of ts-O45-G in ER-exit sites (ERES) and correlates with a reduced mobility of ts-O45-G within ER membranes. A simple mathematical model describing the kinetics of ER-exit predicts that reduced cargo loading to ERES and not the reduced mobility of ts-O45-G accounts for the delayed ER-exit and arrival at the Golgi. Consistent with this, membrane turnover of the COPII component Sec23p is delayed in sterol-depleted cells. Altogether, our results demonstrate the importance of sterol levels in COPII mediated ER-export. IS - 13 JF - The EMBO Journal SN - 0261-4189 EP - 2965 TI - Sterols regulate {ER}-export dynamics of secretory cargo protein {ts-O45-G} PB - Nature Publishing Group VL - 25 SP - 2953 KW - cholesterol KW - copii KW - er KW - sterol KW - vesicle AU - Runz, Heiko AU - Miura, Kota AU - Weiss, Matthias AU - Pepperkok, Rainer PY - 2006/06/22/ UR - http://dx.doi.org/10.1038/sj.emboj.7601205 ER -