| |
Abstract
Near-infrared (NIR) spectroscopy is based on the absorption of electromagnetic radiation at wavelengths in the range 780–2500 nm. NIR spectra of foods comprise broad bands arising from overlapping absorptions corresponding mainly to overtones and combinations of vibrational modes involving CH, OH and NH chemical bonds. The concentrations of constituents such as water, protein, fat and carbohydrate can in principle be determined using classical absorption spectroscopy. However, for most food samples, this chemical information is obscured by changes in the spectra caused by ...
|
| |
posted to mouse nir
by jsgalan
on 2012-12-12 18:22:44
Abstract
The aim of the study was to determine the accuracy of near-infrared spectroscopy (NIRS) in determining species, gender, age, and the presence of the common endosymbiont Wolbachia in laboratory-reared Drosophila. NIRS measures the absorption of light by organic molecules. Initially, a calibration model was developed for each study. An independent set with flies not involved in initial cross-validation was then used to validate the accuracy ...
|
| |
Abstract
To determine if metabolomic profiling of embryonic development was associated with implantation rates in IVF. Prospective blinded. University-affiliated assisted reproductive technology program. Unselected assisted reproductive technology population. None. Raman-based biospectroscopic metabolomic profiling of spent culture media and delivery rates. Forty-one spent media samples from 19 patients with known reproductive potential (0 or 100% delivery rates of each embryo that implanted) were evaluated. Raman-based metabolomic profiling was used to calculate a viability index for each sample. On day 3, the spent media ...
|
| |
posted to mouse nir
by jsgalan
on 2012-12-11 18:48:05
Abstract
Near-infrared calibration models are described for the measurement of total protein, albumin protein, globulin protein, triglycerides, cholesterol, urea, glucose, and lactate. Spectra are collected in triplicate over the 5000–4000 cm−1 spectral range with a 2.5 mm optical path length for 242 undiluted human serum samples. Calibration models are generated for each analyte by performing partial least-squares (PLS) regression on raw and digitally filtered spectra. Models are optimized individually for each analyte by considering spectral range, number of model factors and width/position of a ...
|
| |
posted to mouse nir
by jsgalan
on 2012-12-11 18:44:35
Abstract
STUDY QUESTION: Is the selection of a single Day 3 embryo by metabolomic profiling of culture medium with near-infrared (NIR) spectroscopy as an adjunct to morphology able to improve live birth rates in IVF, compared with embryo selection by morphology alone? SUMMARY ANSWER: The live birth rate after embryo selection by NIR spectroscopy and morphology is not significantly different compared with the live birth rate ...
|
| |
by Carlijn G. Vergouw, Lucy L. Botros, Kevin Judge, et al.Mark Henson, Pieter Roos, E. Hanna Kostelijk, Roel Schats, Jos W. Twisk, Peter G. Hompes, Denny Sakkas, Cornelis B. Lambalk
posted to mouse nir
by jsgalan
on 2012-12-11 18:43:59
Abstract
This study investigated if metabolomic profiling of culture media using near infrared (NIR) spectroscopy was related to live-birth rates after single-embryo transfer of frozen-thawed embryos. Analysis of culture media of frozen-thawed embryos was performed by NIR spectroscopy. A viability score was calculated using a predictive multivariate algorithm of fresh day-5 embryos with known pregnancy outcomes. This algorithm generated with fresh day-5 embryos could help to ...
|
| |
posted to mouse nir
by jsgalan
on 2012-12-11 18:43:08
Abstract
Near infrared (NIR) spectroscopy is a technology proposed to facilitate non-invasive screening for the most optimal human embryo for uterine transfer. It has been proposed that the NIR spectral profile of an embryo's spent culture medium can be used to generate a viability score that correlates to implantation potential. As the initial proof of principle studies were all retrospective, our aim was to investigate whether ...
|
| |
Appl. Spectrosc., Vol. 46, No. 10. (1 October 1992), pp. 1575-1578
posted to mouse
by jsgalan
on 2012-12-11 18:35:50
Abstract
Noninvasive monitoring of glucose in diabetic patients is feasible with the use of near-infrared spectroscopic measurements. As a step toward the final goal of the development of a noninvasive monitor, the near-infrared spectra (4250 to 6600 cm−1) of glucose-doped whole blood samples were obtained along with reference glucose values. Glucose concentrations and spectra of blood samples obtained from four subjects were subjected to multivariate calibration with the use of partial least-squares (PLS) methods. The cross-validated PLS standard errors of prediction for ...
|
| |
posted to mouse
by jsgalan
on 2012-12-11 18:31:02
Abstract
The relative potential of various technologies for the confirmation of food authenticity and quality are discussed. Techniques that have found new applications in the field of quality assurance since 2001 are discussed in terms of their potential ease of application in an industrial setting. The use of specific techniques with chemometric analysis for the classification of food samples based on quality attributes is also included in this review. The techniques discussed are spectroscopy (UV, NIR, MIR, visible, Raman), isotopic analysis, chromatography, ...
|
| |
posted to mouse
by jsgalan
on 2012-12-11 18:26:00
Abstract
Near infrared is a rapid, non-destructive method of analysis which requires no sample preparation. The identification of pure powdered drugs and the active drug in tablets from a database of 96 pure drugs is demonstrated. Powdered pure drugs contained in glass vials and tablets were scanned by placing the samples directly onto the quartz window of a NIRSystems model 6500 near infrared spectrometer. Sampling time was approximately 40 s. The data were analysed using a number of chemometric procedures such as ...
|
| |
Abstract
Bacterial vaginosis (BV) is a highly prevalent vaginal infection that is associated with adverse pregnancy outcomes. Vitamin D exerts an influence on the immune system and may play a role in BV. The objective of this study was to examine the association between maternal vitamin D status and the prevalence of BV in early pregnancy. Women (n = 469) enrolled in a pregnancy cohort study ...
|
| |
posted to mouse
by jsgalan
on 2012-12-11 17:54:31
Abstract
The development of Near Infrared Spectroscopy has paralleled that of the PC, and the application of NIR in many industries has undergone explosive growth in recent years. This has been particularly apparent in the area of microbial and cell culture system monitoring and control. Potentially, NIR offers the prospect of real-time control of the physiology of cultured cells in fermenters, leading to marked improvements in ...
|
| |
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, Vol. 28, No. 6. (2012), pp. 545-563
posted to mouse nir
by jsgalan
on 2012-12-04 19:21:12
Abstract
The purpose of this review article is to outline recent progress in near-infrared (NIR) spectroscopy. Some particular emphasis is put on the delineation of its versatility in analytical chemistry. NIR spectroscopy is versatile in many aspects. For example, it is electronic spectroscopy as well as vibrational spectroscopy. It is also all-round in applications from basic to practical applications. NIR spectroscopy can be applied to various ...
|
| |
posted to mouse nir spectra
by jsgalan
on 2012-12-02 01:29:32
Abstract
Near infrared (NIR) spectroscopy has increasingly been adopted as an analytical tool in various fields, such as the petrochemical, pharmaceutical, environmental, clinical, agricultural, food and biomedical sectors during the past 15 years. A NIR spectrum of a sample is typically measured by modern scanning instruments at hundreds of equally spaced wavelengths. The large number of spectral variables in most data sets encountered in NIR spectral chemometrics often renders the prediction of a dependent variable unreliable. Recently, considerable effort has been directed ...
|
| |
Appl. Spectrosc., Vol. 51, No. 5. (1 May 1997), pp. 631-635
posted to nir spectra
by jsgalan
on 2012-12-02 01:28:27
Abstract
The feasibility of applying molecular spectroscopy in the mid-infrared region to the quantitation of glucose and cholesterol in whole blood and in blood serum was examined by measuring the samples in the dry state on a polyethylene carrier. The partial least-squares (PLS) algorithm was employed to set up the calibration models. Analysis was performed by normalizing the spectra to a standard area. Evidence that this approach is warranted was obtained through experiments where potassium thiocyanate served as the internal standard. The ...
|
| |
posted to mouse nir
by jsgalan
on 2012-12-02 01:27:09
|
| |
Clinical chemistry, Vol. 45, No. 9. (September 1999), pp. 1530-1535
posted to nir
by jsgalan
on 2012-11-30 19:53:45
Abstract
Practical improvements are needed to allow measurement of glucose concentrations by Fourier- transform infrared (FT-IR) spectroscopy. We developed a new method that allows determination of the glucose concentration in dried sera. We studied 32 serum samples after fourfold dilution and desiccation before FT-IR analyses on a spectrometer operated at a ...
|
| |
Pediatriia, No. 7. (July 1979), pp. 13-16
posted to preterm
by jsgalan
on 2012-11-30 19:50:56
|
| |
posted to mouse nir
by jsgalan
on 2012-11-30 19:29:19
Abstract
The infrared spectrum of a mixture serves as the basis to quantitate its constituents, and a number of common clinical chemistry tests have proven to be feasible using this approach. This article reviews the infrared spectroscopy-based analytical methods that have been developed for consideration as clinical assays, including serum analysis, urine analysis, amniotic fluid assays for the estimation of fetal lung maturity, and others. Because of the widespread interest in the potential for in vivo measurement of blood glucose using near-infrared ...
|
| |
Gut, Vol. 30, No. 10. (October 1989), pp. 1344-1347
posted to mouse
by jsgalan
on 2012-11-28 21:18:51
Abstract
This investigation was aimed at comparing a new method for measuring faecal fat excretion, carried out with a semi-automated instrument by using near infrared analysis (NIRA), with the traditional titrimetric (Van de Kamer) and gravimetric (Sobel) methods. Near infrared analysis faecal fat was assayed on the three day stool collection from 118 patients (68 chronic pancreatitis, 19 organic diseases of the gastrointestinal tract, 19 alcoholic ...
|
| |
posted to mouse nir
by jsgalan
on 2012-11-28 20:26:36
Abstract
Platelet (PLT) transfusion-associated bacterial sepsis has remained a substantial patient risk, primarily due to lacking effective and point-of-issue measures to detect bacterial contamination. This study describes near infrared (NIR) spectroscopy to examine inoculated PLTs without sampling within a few seconds. This study evaluated apheresis PLTs inoculated with low concentrations of ...
|
| |
Abstract
In recent years, near-infrared (NIR) spectroscopy has gained wide acceptance in different fields by virtue of its advantages over other analytical techniques, the most salient of which is its ability to record spectra for solid and liquid samples with no prior manipulation. Also, developments in instrumentation have resulted in the manufacture of spectrophotometers capable of quickly providing spectra that are flexible enough for use in different situations; thus, portable equipment can record spectra on site or even at production lines. This ...
|
| |
posted to mouse nir
by jsgalan
on 2012-11-28 19:51:34
Abstract
Metabolic profiling holds promise with regard to deepening our understanding of infection biology and disease states. The objectives of our study were to assess the global metabolic responses to an Echinostoma caproni infection in the mouse, and to compare the biomarkers extracted from different biofluids (plasma, stool, and urine) in terms of characterizing acute and chronic stages of this intestinal fluke infection. ...
|
| |
posted to infection nutrition
by jsgalan
on 2012-11-27 16:12:49
Abstract
▪ Abstract Infection leads to profound alterations in whole-body metabolism, which is characterized by marked acceleration of glucose, fat and protein, and amino acid flux. One of the complications of infection, especially in the nutritionally supported setting, is hyperglycemia. The hyperglycemia is caused by peripheral insulin resistance and alterations in hepatic glucose metabolism. The defects in hepatic glucose metabolism include overproduction of glucose and a failure of the liver to appropriately adapt when nutritional support is administered. Investigators have suggested that ...
|
| |
posted to af mouse
by jsgalan
on 2012-11-22 20:17:50
Abstract
Murine pregnancy is characterised by marked increases in serum cytokine profiles with advancing gestation, but whether these changes reflect concentrations in amniotic fluid is unknown. This study therefore profiled 23 cytokines by fluid-phase multiplex immunoassay of amniotic fluid and serum collected from naturally mated mice during mid- and late pregnancy (days 11 and 18, respectively). The marked increase in serum profile of many cytokines from ...
|
| |
posted to af mouse preterm
by jsgalan
on 2012-11-22 19:53:37
Abstract
Objective: The aim of this study was to attempt to correlate the quantitative data obtained from the near-infrared (near-IR) spectra of amniotic fluid with the lecithin/sphingomyelin () ratio determined from thin layer chromatographic analysis on the same samples, in order to establish the feasibility of near-IR spectroscopy as an alternate method for the prediction of fetal lung development. Methods: Samples of amniotic fluid were obtained by amniocentesis from 52 patients between the 26th and 41st week of pregnancy. About 350 μl ...
|
| |
posted to biomedical chemometrics
by jsgalan
on 2012-11-22 19:24:58
Abstract
The use of chemometrics in quantitative near-infrared (NIR) spectroscopy is reviewed from the standpoint of avoiding pitfalls that may lead to misleading or overly optimistic results. Using the NIR analysis of glucose in six-component mixture samples as an example, a set of guidelines is presented to help the analyst develop and implement a successful calibration. ...
|
| |
Abstract
Both infrared (IR) and Raman spectroscopy are emerging as powerful probes of biomedically relevant properties of tissue and biological fluids. From tentative first steps, this field of endeavor is now beginning to mature as the central conceptual and technical issues come into focus. Using representative examples mainly from our own research, the aim of the present article is to provide the reader with a brief overview of progress to date. ...
|
| |
posted to mouse
by jsgalan
on 2012-11-22 18:56:07
Abstract
This is a review of the emerging role of infrared spectroscopy in medical research and practice, illustrated with representative examples from the Institute for Biodiagnostics and from the literature. ...
|
| |
Clinical chemistry, Vol. 38, No. 9. (September 1992), pp. 1623-1631
posted to mouse
by jsgalan
on 2012-11-22 18:53:34
Abstract
The near-infrared (NIR) spectral region (700-2500 nm) is a fertile source of chemical information in the form of overtone and combination bands of the fundamental infrared absorptions and low-energy electronic transitions. This region was initially perceived as being too complex for interpretation and consequently was poorly utilized. Advances in chemometric techniques that can extract massive amounts of chemical information from the highly overlapped, complex spectra ...
|
| |
posted to mouse
by jsgalan
on 2012-11-22 16:14:09
Abstract
Although several methods, including enzyme-linked immunosorbent assay, polymerase chain reaction, immunofluorescent assay, and Western blotting, have been used for the diagnosis of viral infections, none of them is ideal in terms of cost-effectiveness, speed, and accuracy. Currently, the rate of outbreak of emerging viruses is increasing and therefore the development and establishment of analytical methods for such viral infections are becoming more important. Near-infrared (NIR) spectroscopy is a fast, multicomponent assay that enables non-invasive, non-destructive analysis. Recently, the diagnosis of viral ...
|
| |
J. Proteome Res. In Journal of Proteome Research, Vol. 10, No. 4. (6 January 2011), pp. 1437-1448, doi:10.1021/pr101067u
posted to mouse
by jsgalan
on 2012-11-22 16:12:06
along with 1 person
dguldag
Abstract
Biospectroscopy is employed to derive absorbance spectra representative of biomolecules present in biological samples. The mid-infrared region (? = 2.5 ?m?25 ?m) is absorbed to give a biochemical-cell fingerprint (v? = 1800?900 cm?1). Cellular material produces complex spectra due to the variety of chemical bonds present. The complexity and size of spectral data sets warrant multivariate analysis for data reduction, interpretation, and classification. Various multivariate analyses are available including principal component analysis (PCA), partial least-squares (PLS), linear discriminant analysis (LDA), and ...
|
| |
Veterinary parasitology, Vol. 84, No. 3-4. (1 August 1999), pp. 187-204
posted to mouse
by jsgalan
on 2012-11-20 19:29:53
Abstract
The interactions between host nutrition and parasitism in ruminants are viewed within a framework that accounts for the allocation of scarce nutrient resources, such as energy and protein, between the various competing body functions of the host. These include functions that are the direct result of parasitism. Since it is proposed that the host gives priority to the reversal of the pathophysiological consequences of parasitism ...
|
| |
posted to mouse
by jsgalan
on 2012-11-20 19:26:45
Abstract
Nutritionists have long understood that intestinal nematode parasites have deleterious effects on host nutritional status, but only recently has the importance of malnutrition as a predisposing factor to intestinal nematodes been recognized. Here we review experimental and field studies on the effects of protein, energy, zinc, vitamin A, and iron deficiencies on gastrointestinal (GI) nematodes of humans, livestock, and laboratory rodents, and draw certain conclusions ...
|
| |
posted to mouse
by jsgalan
on 2012-11-20 18:49:53
Abstract
In this work we propose a rapid method based on visible and near-infrared (Vis-NIR) spectroscopy to determine the occurrence of double-stranded RNA (dsRNA) viruses in Epichloë festucae strains isolated from Festuca rubra plants. In addition, we examined the incidence of infections by E. festucae in populations of F. rubra collected in natural grasslands of Western Spain. ...
|
| |
posted to mouse
by jsgalan
on 2012-11-20 07:26:31
Abstract
Obesity, whose prevalence is increasing rapidly worldwide, is recognized as a risk factor for diabetes, cardiovascular disease, liver disease, and renal disease. To investigate metabolic changes in the urine of a rat model of obesity induced by a high-fat diet (HFD), rats were divided into the following four groups based on the diet type and degree of weight gain: normal-diet (ND) low gainers, ND high ...
|
| |
posted to mouse
by jsgalan
on 2012-11-20 07:15:31
Abstract
BACKGROUND: Influenza patients show a severe condition of the respiratory tract with high temperature. Efficient treatment of influenza requires early use of oseltamivir, and thus rapid diagnosis is needed. Recently, rapid diagnostic methods such as immunochromatography have been developed; however, immunochromatography is not an optimal technique because it is relatively expensive and has low sensitivity. METHODS: Visible and near-infrared (Vis-NIR) spectroscopy in the region 600-1100nm, ...
|
| |
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, Vol. 55, No. 4. (December 2012), pp. 334-338, doi:10.1016/j.jcv.2012.08.015
posted to mouse
by jsgalan
on 2012-11-20 07:10:43
Abstract
Human influenza A and B viruses cause severe seasonal respiratory tract infections, especially in infants and young children. Influenza A and B viruses have been reported to produce different symptoms and/or severity in infected patients, although these remain inconclusive. In this study, non-invasive visible and near-infrared (Vis-NIR) spectroscopy was used ...
|
| |
Clinica chimica acta; international journal of clinical chemistry, Vol. 413, No. 3-4. (18 February 2012), pp. 467-472, doi:10.1016/j.cca.2011.10.035
posted to mouse
by jsgalan
on 2012-11-20 07:08:54
Abstract
Previously, we investigated the possibility of using near-infrared (NIR) spectroscopy for the diagnosis of human immunodeficiency virus type-1 (HIV-1) infection. Here, we further analyze NIR spectra using molecular clones of various HIV-1 subtypes. Culture supernatants of pNL4-3- (HIV-1 molecular clone) or pUC18- (empty vector) transfected 293 T cells were used. ...
|
| |
Biotechnology and bioengineering, Vol. 77, No. 1. (5 January 2002), pp. 73-82
posted to no-tag
by jsgalan
on 2012-11-12 22:11:57
Abstract
An adaptive calibration procedure is used to build selective multivariate calibration models for the measurement of glucose, lactate, glutamine, and ammonia in undiluted serum-based cell culture media. This adaptive procedure removes metabolism-induced covariance between these analytes in a series of calibration samples collected during the cultivation of PC-3 human prostate cancer cells. Partial least-squares calibration models are generated from single-beam near-infrared (NIR) spectra collected over ...
|
| |
posted to mouse
by jsgalan
on 2012-11-11 20:21:04
|
| |
posted to af mouse
by jsgalan
on 2012-11-11 19:45:06
Abstract
This pilot study investigated the possibility that metabolomic differences exist in second trimester of women delivering at term (≥37 weeks, n = 216) and preterm (≤35 weeks, n = 11). For this retrospective study, biobanked AF samples underwent near-infrared (NIR) spectral analysis using wavelengths from 700 to 1050 nm. Spectral data was compressed then optimized by multilinear regression to create a calibration model. The resultant model was able to classify term and preterm births based on differing AF metabolomic profiles with a ...
|
| |
posted to mouse
by jsgalan
on 2012-08-31 17:29:43
Abstract
Using a 2 times 2 factorial design, we investigated the combined impact of protein deficiency (PD) and gastrointestinal nematode infection during late pregnancy and lactation on resting metabolic rate (RMR), body composition and bone mineralization, neonatal growth, and the regulatory hormones [corticosterone, leptin, and insulin-like growth factor-1 (IGF-1)] and proinflammatory cytokines [interleukin (IL)-1 beta and IL-6] that may drive these processes. Pregnant CD1 mice, fed either a protein-sufficient (PS; 24%) or protein-deficient (PD; 6%) isocaloric diet, were infected 4 times with ...
|
| |
posted to mouse
by jsgalan
on 2012-08-31 17:12:41
Abstract
This study examined concurrent stresses of nematode infection and pregnancy using pregnant and non-pregnant CD1 mice infected 3 times with 0, 50 or 100 Heligmosomoides bakeri larvae. Physiological, energetic, immunological and skeletal responses were measured in maternal and foetal compartments. Resting metabolic rate (RMR) was elevated by pregnancy, but not by the trickle infection. Energy demands during pregnancy were met through increased food intake and fat utilization whereas mice lowered their body temperature during infection. Both infection and pregnancy increased visceral ...
|
| |
Clinical Infectious Diseases, Vol. 46, No. 10. (15 May 2008), pp. 1582-1588, doi:10.1086/587658
Abstract
Infection and malnutrition have always been intricately linked. Malnutrition is the primary cause of immunodeficiency worldwide, and we are learning more and more about the pathogenesis of this interaction. Five infectious diseases account for more than one-half of all deaths in children aged <5 years, most of whom are undernourished. Micronutrient deficiencies have effects such as poor growth, impaired intellect, and increased mortality and susceptibility to infection. The worldwide magnitude of parasite infection is enormous. It is understood that parasites may ...
|
| |
posted to mouse
by jsgalan
on 2012-07-09 18:48:11
Abstract
The current study was undertaken to determine simultaneous changes in amniotic fluid (AF) volume and composition across gestation in the pregnant mouse. Young adult mice (6 to 7 weeks old) of the CB6F1 strain were mated overnight. AF was collected on consecutive days from embryonic days 9.5 through 18.5 for measurements of volume and composition. Statistical analysis included one-factor analysis of variance (ANOVA). ...
|
| |
posted to oxidative_stress
by jsgalan
on 2012-07-03 16:11:44
Abstract
Oxidative stress is widely implicated in failed reproductive performance, including infertility, miscarriage, diabetes-related congenital malformations, and preeclampsia. Maternal obesity is a strong risk factor for preeclampsia, and in a recent study we observed oxidative stress in the oocytes of obese animals before pregnancy as well as in early-stage embryos. This adds to the growing evidence that investigators need to focus more on the preconceptual period ...
|
| |
posted to nir
by jsgalan
on 2012-06-11 22:45:11
Abstract
Near-infrared (NIR) spectra in the region of 5000–4000 cm−1 with a chemometric method called searching combination moving window partial least squares (SCMWPLS) were employed to determine the concentrations of human serum albumin (HSA), γ-globulin, and glucose contained in the control serum IIB (CS IIB) solutions with various concentrations. SCMWPLS is proposed to search for the optimized combinations of informative regions, which are spectral intervals, considered containing useful information for building partial least squares (PLS) models. The informative regions can easily be found ...
|
| |
posted to mouse nir
by jsgalan
on 2012-06-11 22:06:42
Abstract
We have determined total protein, albumin, globulins, and urea in unmodified human serum using near-infrared (NIR) spectroscopy. The distinctive NIR spectrum of the individual protein fractions allowed unique spectroscopic models to be derived for each fraction using multiple linear least-squares regression (MLR). A more sophisticated partial least-squares (PLS) regression analysis was necessary to fully extract the relevant spectral information for urea. The serum protein data sets consisted of 235 calibration samples and 85 prediction samples. The total protein calibration yielded a ...
|
| |
Clinical and experimental immunology, Vol. 41, No. 2. (August 1980), pp. 303-308
posted to ce_cordblood
by jsgalan
on 2012-05-09 15:18:28
Abstract
Total IgG concentrations and the concentrations of the four subclasses of IgG were estimated in thirty-four pairs of maternal and foetal sera from pregnancies of various gestations ranging from 28 to 42 weeks using the method of radial immunodiffusion. It was found that: (1) all subclasses of IgG cross the human placenta freely, (2) foetal levels of IgG and each subclass of IgG exceed maternal ...
|
Search
