Surface imprinted beads for the recognition of human serum albumin.

@article{citeulike:1445294, abstract = {The synthesis of poly-aminophenylboronic acid (ABPA) imprinted beads for the recognition of the protein human serum albumin (HSA) is reported. In order to create homogeneous recognition sites, covalent immobilisation of the template HSA was exploited. The resulting imprinted beads were selective for HSA. The indirect imprinting factor (IF) calculated from supernatant was 1.6 and the direct IF, evaluated from the protein recovered from the beads, was 1.9. The binding capacity was 1.4 mg/g, which is comparable to commercially available affinity materials. The specificity of the HSA recognition was evaluated with competitive experiments, indicating a molar ratio 4.5/1 of competitor was necessary to displace half of the bound HSA. The recognition and binding of the imprinted beads was also tested with a complex sample, human serum and targeted removal of HSA without a loss of the other protein components was demonstrated. The easy preparation protocol of derivatised beads and a good protein recognition properties make the approach an attractive solution to analytical and bio-analytical problems in the field of biotechnology.}, address = {Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy.}, author = {Bonini, F. and Piletsky, S. and Turner, A. P. and Speghini, A. and Bossi, A. }, citeulike-article-id = {1445294}, doi = {10.1016/j.bios.2006.12.034}, issn = {0956-5663}, journal = {Biosens Bioelectron}, keywords = {albumin, method}, month = {April}, number = {9-10}, pages = {2322--2328}, posted-at = {2007-07-10 04:23:37}, priority = {2}, title = {Surface imprinted beads for the recognition of human serum albumin.}, url = {http://dx.doi.org/10.1016/j.bios.2006.12.034}, volume = {22}, year = {2007} }

TY - JOUR ID - citeulike:1445294 L3 - citeulike-article-id:1445294 TI - Surface imprinted beads for the recognition of human serum albumin. JF - Biosens Bioelectron VL - 22 IS - 9-10 SP - 2322 EP - 2328 AD - Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy. SN - 0956-5663 N2 - The synthesis of poly-aminophenylboronic acid (ABPA) imprinted beads for the recognition of the protein human serum albumin (HSA) is reported. In order to create homogeneous recognition sites, covalent immobilisation of the template HSA was exploited. The resulting imprinted beads were selective for HSA. The indirect imprinting factor (IF) calculated from supernatant was 1.6 and the direct IF, evaluated from the protein recovered from the beads, was 1.9. The binding capacity was 1.4 mg/g, which is comparable to commercially available affinity materials. The specificity of the HSA recognition was evaluated with competitive experiments, indicating a molar ratio 4.5/1 of competitor was necessary to displace half of the bound HSA. The recognition and binding of the imprinted beads was also tested with a complex sample, human serum and targeted removal of HSA without a loss of the other protein components was demonstrated. The easy preparation protocol of derivatised beads and a good protein recognition properties make the approach an attractive solution to analytical and bio-analytical problems in the field of biotechnology. KW - albumin KW - method AU - Bonini, F AU - Piletsky, S AU - Turner, AP AU - Speghini, A AU - Bossi, A PY - 2007/04/15/ UR - http://dx.doi.org/10.1016/j.bios.2006.12.034 ER -