Vitamin K Epoxide Reductase: A Protein Involved in Angiogenesis Export

@article{citeulike:615677, abstract = {10.1158/1541-7786.MCR-04-0221 Vitamin K epoxide reductase (VKOR) is a newly identified protein which has been reported to convert the epoxide of vitamin K back to vitamin K, a cofactor essential for the posttranslational γ-carboxylation of several blood coagulation factors. We found that the gene is expressed ubiquitously including vascular endothelial cells, smooth muscle cells, fibroblasts and cardiomyocytes, and is overexpressed in 11 tumor tissues on microarray. Stable transfection of VKOR cDNA into tumor cell line A549 and H7402 did not promote the cell proliferation. These results promoted us to hypothesize that VKOR may also be involved in angiogenesis. To test this hypothesis, the expression of VKOR was studied in different vascular cells in developmental and pathologic heart tissues. The effects of overexpression and suppressing expression of VKOR on endothelial cell proliferation, migration, adhesion, and tubular network formation were explored. We found that VKOR expression in arteries was prominent in vascular endothelial cells and was high in the ventricular aneurysm tissue of human heart and human fetal heart. studies showed that overexpression of VKOR slightly but significantly stimulated human umbilical vein endothelial cell proliferation (by 120\%), migration (by 118\%), adhesion (by 117\%), as well as tubular network formation. Antisense to gene inhibited the proliferation (by 67\%), migration (by 64\%), adhesion (by 50\%), and tubular network formation. Our findings support the impact of VKOR in the process of angiogenesis; hence, the molecule may have a potential application in cardiovascular disease and cancer therapy.}, address = {Sino-German Laboratory for Molecular Medicine and Hypertension Division, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Beilishilu, Xicheng District, Beijing 100037, P.R. China.}, author = {Wang, Yibo and Zhen, Yisong and Shi, Yi and Chen, Jingzhou and Zhang, Channa and Wang, Xiaojian and Yang, Xu and Zheng, Yi and Liu, Yuqing and Hui, Rutai}, citeulike-article-id = {615677}, citeulike-linkout-0 = {http://dx.doi.org/10.1158/1541-7786.MCR-04-0221}, citeulike-linkout-1 = {http://mcr.aacrjournals.org/content/3/6/317.abstract}, citeulike-linkout-2 = {http://mcr.aacrjournals.org/content/3/6/317.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/15972850}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=15972850}, comment = {楠田 06/05/17 1回目}, doi = {10.1158/1541-7786.MCR-04-0221}, issn = {1541-7786}, journal = {Molecular Cancer Research}, keywords = {angiogenesis, seminar, vitamink, vkepo-reductase}, month = {June}, number = {6}, pages = {317--323}, posted-at = {2006-05-06 08:06:25}, priority = {0}, title = {Vitamin K Epoxide Reductase: A Protein Involved in Angiogenesis}, url = {http://dx.doi.org/10.1158/1541-7786.MCR-04-0221}, volume = {3}, year = {2005} }

TY - JOUR ID - citeulike:615677 L3 - citeulike-article-id:615677 N2 - 10.1158/1541-7786.MCR-04-0221 Vitamin K epoxide reductase (VKOR) is a newly identified protein which has been reported to convert the epoxide of vitamin K back to vitamin K, a cofactor essential for the posttranslational γ-carboxylation of several blood coagulation factors. We found that the gene is expressed ubiquitously including vascular endothelial cells, smooth muscle cells, fibroblasts and cardiomyocytes, and is overexpressed in 11 tumor tissues on microarray. Stable transfection of VKOR cDNA into tumor cell line A549 and H7402 did not promote the cell proliferation. These results promoted us to hypothesize that VKOR may also be involved in angiogenesis. To test this hypothesis, the expression of VKOR was studied in different vascular cells in developmental and pathologic heart tissues. The effects of overexpression and suppressing expression of VKOR on endothelial cell proliferation, migration, adhesion, and tubular network formation were explored. We found that VKOR expression in arteries was prominent in vascular endothelial cells and was high in the ventricular aneurysm tissue of human heart and human fetal heart. studies showed that overexpression of VKOR slightly but significantly stimulated human umbilical vein endothelial cell proliferation (by 120%), migration (by 118%), adhesion (by 117%), as well as tubular network formation. Antisense to gene inhibited the proliferation (by 67%), migration (by 64%), adhesion (by 50%), and tubular network formation. Our findings support the impact of VKOR in the process of angiogenesis; hence, the molecule may have a potential application in cardiovascular disease and cancer therapy. IS - 6 JF - Molecular Cancer Research SN - 1541-7786 AD - Sino-German Laboratory for Molecular Medicine and Hypertension Division, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Beilishilu, Xicheng District, Beijing 100037, P.R. China. EP - 323 TI - Vitamin K Epoxide Reductase: A Protein Involved in Angiogenesis VL - 3 SP - 317 KW - angiogenesis KW - seminar KW - vitamink KW - vkepo-reductase N1 - 楠田 06/05/17 1回目 AU - Wang, Yibo AU - Zhen, Yisong AU - Shi, Yi AU - Chen, Jingzhou AU - Zhang, Channa AU - Wang, Xiaojian AU - Yang, Xu AU - Zheng, Yi AU - Liu, Yuqing AU - Hui, Rutai PY - 2005/06// UR - http://dx.doi.org/10.1158/1541-7786.MCR-04-0221 ER -