Technetium labeling of dextran incorporating cysteamine as a ligand. Export

@article{citeulike:625153, abstract = {INTRODUCTION: Technetium-99m-labeled dextran is a useful imaging agent for procedures such as angiocardiography and lymphoscintigraphy. To improve the availability of 99mTc-labeled dextran, we designed a cysteamine ligand system for dextran labeling. METHODS: Cysteamine derivatized dextran was synthesized as follows. Dextran was oxidized with sodium periodate, coupled with cysteamine and reduced with sodium borohydride to provide the desired amine ligand. The cysteamine-dextran conjugate was then labeled with reduced 99mTc. Whole-body scintigraphy and biodistribution were examined following injection of the 99mTc-labeled cysteamine-conjugated dextran (99mTc-cysteamine-dextran) in ICR mice. Lymphoscintigraphy was performed after intradermal injection of 99mTc-cysteamine-dextran in SD rats. RESULTS: The cysteamine-derived dextran was easily labeled with reduced 99mTc in greater than 96\% yield. 99mTc-cysteamine-dextran has a higher chelation stability against diethylenetriamine pentaacetic acid (DTPA) than the 99mTc-dextran. Axillary lymph nodes were clearly visible after intradermal injection of 99mTc-cysteamine-dextran in rats. CONCLUSION: These results suggest that 99mTc-cysteamine-dextran is available for lymphoscintigraphy. This methodology could expand the usage of 99mTc-labeled dextran, particularly for diagnostic purposes.}, address = {Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan. k-matsu@fukuoka-u.ac.jp}, author = {Matsunaga, K. and Hara, K. and Imamura, T. and Fujioka, T. and Takata, J. and Karube, Y.}, citeulike-article-id = {625153}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.nucmedbio.2004.12.007}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15820763}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15820763}, doi = {10.1016/j.nucmedbio.2004.12.007}, issn = {0969-8051}, journal = {Nucl Med Biol}, keywords = {dextran, technetium}, month = {April}, number = {3}, pages = {279--285}, posted-at = {2006-05-12 14:41:00}, priority = {0}, title = {Technetium labeling of dextran incorporating cysteamine as a ligand.}, url = {http://dx.doi.org/10.1016/j.nucmedbio.2004.12.007}, volume = {32}, year = {2005} }

TY - JOUR ID - citeulike:625153 L3 - citeulike-article-id:625153 N2 - INTRODUCTION: Technetium-99m-labeled dextran is a useful imaging agent for procedures such as angiocardiography and lymphoscintigraphy. To improve the availability of 99mTc-labeled dextran, we designed a cysteamine ligand system for dextran labeling. METHODS: Cysteamine derivatized dextran was synthesized as follows. Dextran was oxidized with sodium periodate, coupled with cysteamine and reduced with sodium borohydride to provide the desired amine ligand. The cysteamine-dextran conjugate was then labeled with reduced 99mTc. Whole-body scintigraphy and biodistribution were examined following injection of the 99mTc-labeled cysteamine-conjugated dextran (99mTc-cysteamine-dextran) in ICR mice. Lymphoscintigraphy was performed after intradermal injection of 99mTc-cysteamine-dextran in SD rats. RESULTS: The cysteamine-derived dextran was easily labeled with reduced 99mTc in greater than 96% yield. 99mTc-cysteamine-dextran has a higher chelation stability against diethylenetriamine pentaacetic acid (DTPA) than the 99mTc-dextran. Axillary lymph nodes were clearly visible after intradermal injection of 99mTc-cysteamine-dextran in rats. CONCLUSION: These results suggest that 99mTc-cysteamine-dextran is available for lymphoscintigraphy. This methodology could expand the usage of 99mTc-labeled dextran, particularly for diagnostic purposes. IS - 3 JF - Nucl Med Biol SN - 0969-8051 AD - Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan. k-matsu@fukuoka-u.ac.jp EP - 285 TI - Technetium labeling of dextran incorporating cysteamine as a ligand. VL - 32 SP - 279 KW - dextran KW - technetium AU - Matsunaga, K AU - Hara, K AU - Imamura, T AU - Fujioka, T AU - Takata, J AU - Karube, Y PY - 2005/04// UR - http://dx.doi.org/10.1016/j.nucmedbio.2004.12.007 ER -